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Hi all, Has anyone perform neutrophil depletion and adoptive transfer experiments? To determine the function of one neutrophil receptor, I performed the following neutrophil adoptive transfer...
28 December 2022 4,870 5 View
I'm doing experiments on neutrophils producing NETs. In the preliminary experiment, I successfully induced NETs from rat neutrophils using PMA. When I used ADP preactivated platelets to stimulate...
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Hi, everyone! I am a graduate student of pharmacology from China. I am trying to measure the plasma NETs level with anti-MPO antibody and Sytox Green, which are available in our lab. Here's...
05 December 2021 703 1 View
Hi all, I read in the literature that when mesure plasma cfDNA with Sytox Green, plasma was diluted with PBS containing 01%BSA. Is this a necessary step? What is the purpose of adding 0.1%BSA....
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I am a master student from China. I need to seperate platelet-rich plasma and count with a light microscope and a blood count plate. I want to know if any method can help me see the platelet...
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