Fang Yafei

6 Questions 7 Answers 0 Followers

Questions related from Fang Yafei

Why do neutrophils disappear so quickly after injection into mice?

Hi all, Has anyone perform neutrophil depletion and adoptive transfer experiments? To determine the function of one neutrophil receptor, I performed the following neutrophil adoptive transfer...

28 December 2022 4,908 5 View

How to induce NETs with activated platelet?

I'm doing experiments on neutrophils producing NETs. In the preliminary experiment, I successfully induced NETs from rat neutrophils using PMA. When I used ADP preactivated platelets to stimulate...

28 February 2022 4,595 7 View

How to detect plasma NETs(MPO-DNA complex) levels by ELISA and Sytox Green?

Hi, everyone! I am a graduate student of pharmacology from China. I am trying to measure the plasma NETs level with anti-MPO antibody and Sytox Green, which are available in our lab. Here's...

05 December 2021 730 1 View

How to measure cfDNA in plasma with Sytox Green?

Hi all， I read in the literature that when mesure plasma cfDNA with Sytox Green, plasma was diluted with PBS containing 01%BSA. Is this a necessary step? What is the purpose of adding 0.1%BSA....

04 October 2021 7,287 0 View

How to preserve anticoagulant venous blood briefly?

In my previous experiment, I keep the anticoagulant blood at 4℃ to prevent the degradation of the active substances in the anticoagulant blood. But, I noticed some clotting in the blood after a...

24 September 2021 4,977 0 View

How to perform platelet count with a light microscope?

I am a master student from China. I need to seperate platelet-rich plasma and count with a light microscope and a blood count plate. I want to know if any method can help me see the platelet...

14 September 2021 7,728 3 View