What is the best way to extract protein form bacterial cells?

Hi Everyone! I have a sample of bacteria cells, and I want to sonicate them to extract cytosolic proteins. I found a protocol using Tris-HCl buffer, but this lab did not use proteases inhibitor....

13 December 2020 2,603 3 View

Soil, water, and air microbiome?

Hi everyone! I am looking for a database that contains a list of organisms, preferentially bacteria, present in the air, water, and soil. Does anyone know any good database/website? Thanks!! Rocio

24 March 2020 2,009 3 View

Why is STZ considered to be inconvenient for type 1 diabetes?

Why is STZ considered to be inconvenient for type 1 diabetes? if when you inject the rats they are not insulin dependent at low doses (45 mg/kg), but if they are hyperglycemic rats. if it were...

19 March 2020 348 4 View

Does anyone know an updated plant pathogen database?

Hi all, I am working on proteomics, and I am looking for a plant pathogen updated database. Most of them are no longer available. Thank you so much!

15 January 2020 7,157 3 View

How education and entrepreneurship is articulated in your country?

In Ecuador, entrepreneurship is in vogue. The country has a legal framework that normalizes the articulation of Education and Entrepreneurship. In addition, Ecuador has the highest rate of Total...

15 July 2019 7,807 6 View

What do you think about the liberation of women in social life?

In these yars wowen are more importat in this sfere of the life

25 February 2019 6,224 6 View

How can muscle weakness of diabetic rats be ruled out if I am going to use the forced swimming test?

to avoid having a bias in the study with diabetic rats, I need to rule out muscle weakness in the forced swimming test or which method I should use to measure the depressive type behavior where...

01 October 2017 270 2 View

TaqMan MicroRNA cell-to-Ct Kit?

Hi! It is my first time using TaqMan kits, so want to know something about Reverse Transcription process. I recently bought this Kit to extract microRNAs, to do the RT and to quantify on qPCR, and...

19 April 2017 5,157 2 View

Has anyone use anti-FLAG antibody for immunohistochemistry in paraffin/cryostat sections?

27 October 2016 3,966 1 View

[SOLVED] How can I solve the "Cannot read FoldX RepairPDB output" error in Yasara/FoldX?

I am trying to model point mutations in protein structures in Yasara using the FoldX plugin but I always get the same error: "Cannot read FoldX RepairPDB output". This is apparently a known error...

05 July 2016 7,716 2 View

How to compare two groups with only two measurements?

Hiiiii everyone! I have an enquiry on statistical analysis. I was looking for many forum and it's still cannot solve my problem. I want to compare means of two groups of data but only with two...

03 March 2021 8,796 3 View

Why should we choose deep learning to machine learning? Why is CNN better for image classification? Can I get Typical Answer?

What Characteristics makes CNN work better?

03 March 2021 1,458 4 View

Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence?

I am on the lookout for the Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence. Does anyone know where I can find it? Thank you in advance

03 March 2021 3,568 1 View

What kind of fluid could I use for a pitfall trap that also does not invalidate molecular testing?

Hi, I want to start testing pitfall trap to obtain ants samples, but I need to conduct molecular analysis on those insects. So, what kind of fluid can I use? Ethanol expires too early and I need...

03 March 2021 5,978 5 View

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10?

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10? Since, the growth curve from day 2 to 10 won't be like the "Logistic curve" it might not follow logistic...

03 March 2021 1,401 3 View

Can we block unwanted images during the x-ray prediction?

i am try to classify the x-ray images. During classification , can i block unwanted images (except x-ray image).

03 March 2021 7,100 1 View

Do we need to write protocol for a second study of a systematic review that has been registered and published?

I have conducted and published a systematic review and meta-analysis research with the topic related to public health and health pomotion (protocol was registed in PROSPERO). Now we want to...

03 March 2021 8,920 3 View

How to increase a model accuracy ?

dear community, my model is based feature extraction from non stationary signals using discrete Wavelet Transform and then using statistical features then machine learning classifiers in order to...

03 March 2021 6,994 5 View

Is there a classification scheme for rock cavities or caverns with respect to their size and shape?

The range of forms and sizes of cavities in rock is very big. Is there a classification scheme for rock cavities regarding their size and shape?

02 March 2021 4,511 3 View

Do I need to do linear regression before running mediation analysis using PROCESS MACRO by Hayes ?

I just wanted to check if I need to run a linear regression separately if I am using PROCESS MACRO to run mediation analysis. Thank you.

02 March 2021 4,359 3 View