How to obtain a complete sequence of a genome using many contigs?

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Gel electrophoresis result for total RNA samples, 1.5 Agarose gel was used, How to improve the integrity of RNA?

Gel electrophoresis, RNA degradation, RNA extraction from fresh tissue

02 March 2021 5,433 5 View

Why my Total RNA samples do not have clear two bands?

This is gel electrophoresis result for total RNA samples with good conc. However, I guess there is a problem with the purity of the samples, i can't figure out why it appears like this? And how...

22 February 2021 338 9 View

Can I make mixture of cinnamic acid with morin flavanoid ?

I am working on a project for assessing the Antibacterial activity of various chemicals, I was wondering Can i make solution of those components using water as a solvent, depending on the fact...

19 November 2020 7,857 4 View

Did anyone used microflow imaging to measure the size distribution of Multivesicular liposome which are charcterized with size (>3um)??

I have prepared Multivesicular liposomes using the following procedures: Multivesicular liposome formulations for the sustained deliv... Multivesicular liposome (MVL) sustained delivery of a...

18 November 2020 3,189 2 View

How antibodies differentiate according to match every epitope?

How antibodies differentiate according to match every epitope , ie: how takes its shape as a monoclonal

10 October 2020 9,573 2 View

I have 4 latent variables and each of which has 7 observed variables in SEM, how this affects the parsimony of the model?

I am conducting an investigation using SEM (Structural Equation Modelling) and I have 4 latent variables and each of which has 7 observed variables. How this affects the parsimony of the model?...

07 September 2020 718 3 View

How to express Nano-fluid in conservation equation of Mass, Momentum and Energy?

07 September 2020 5,903 1 View

How to examine hypothesis in SmartPLS?

Should I report the Path coefficient or Latent variable correlations after bootstrapping as the P value and T-Statistics in the latent variable correlations of two hypotheses are met while in path...

07 September 2020 6,994 2 View

I followed a common procedure to synthesize Fe3O4 nanoparticles. The problem is that XRD pattern does not show any crystal structure. Could you help?

Besides, I have performed different characterization tecniques to my Fe3O4 nanoparticles and all are similar to what is reported in literature. Those characterization techniques include FTIR, SAED...

22 July 2020 8,549 12 View

How much water does each desalination plant type produce and cost?

There are many types of water desalination plants; Reverse Osmosis, MSF, Solar desalination units, ........... etc. How much water does each desalination plant type produce and what is the...

30 May 2020 7,719 4 View

Plasmid amplification in S2 cell?

When co-transfect S2 cell with selection vector (pCoHygro or pCoBlast ) to make a stable cell line, does the gene integrate into the genome or just like E.coli? How do the plasmids amplify within...

09 February 2021 5,423 1 View

How can i improve my DNA purity for whole genome sequence that has high 260/230 ratio ?

I am performing whole genome sequence for gram negative acinetobacter baumannii using two platforms illumina miswq and oxford nanopore. Thus i require a very high yield and high purity. I am using...

31 January 2021 355 2 View

Is there any alternative to BUSCO for searching orthologues within genomes available?

I have a need to search for single-copy conservative orthologs within several plant genomes and used BUSCO tool for that. However, I found that the results produced by different versions on the...

29 January 2021 7,764 6 View

How to annotate list of gene names using Bash/R?

I have a list of gene names from deep sequencing from a constructed CRISPR knockout library. I was hoping to write a script to annotate all the gene names with a quick description of product...

27 January 2021 8,035 1 View

Question regarding investigation of the nature of bacteria: whether it can cause human diseases or not?

Hi all, Considering that one has the whole genome sequence (annotated) of a bacterium and wants to know if it has the potential to cause human diseases, what steps would you recommend...

26 January 2021 8,732 3 View

Can anyone explain why my starters are amplifying sequence from human genome instead of HPV16?

Hello, I'm working on primers from the publication: Okodo M, Okayama K, Teruya K, Sasagawa T. Uniplex E6/E7 PCR method detecting E6 or E7 genes in 39 human papillomavirus types. J Med Virol....

24 January 2021 7,636 3 View

What is your raw statistical treatment preference?

Hi, I have raw data from [HG-U133_Plus_2] Affymetrix Human Genome U133 Plus 2.0 Array that I want to process using the Expresso function for Affymetrix microarrays. My samples include tumor...

22 January 2021 1,527 3 View

Can use Unigenes from RNA sequences for overexpression studies (transformation) without requiring a specific plant genome?

Dear researchers: If the plant genome has not been released yet, can Unigenes from RNA sequences be used after BLAST and confirmed with known Arabidopsis genes for function/overexpression...

20 January 2021 5,557 2 View

Can BLAST/ search by using known Domain in Transcriptome/Genome data for a gene family?

Dear Researchers, I have Transcriptome/Genome data and want to list all the member of a KCS/CER gene family whose Domain AP2/ERF, FAE1_CUT1, according to the Arabidopsis database, so is there...

15 January 2021 2,293 3 View

When making a phylogenetic tree, do I need to concatenate the chromosome and the plasmids for my reference?

This is such a basic question but I come from a non-bioinfo group so I keep getting different answers. I was wondering if when making a phylogenetic tree whether you have to concatenate the...

14 January 2021 2,492 2 View

Paul Rutland

you could look at CAP3 described below

CAP3 Sequence Assembly Program Enter your sequences in FASTAformat (no more than 50 kb): This form allows you to assemble a set of contiguous sequences (contigs) with the CAP3program. If you use CAP3 in any published work, please cite the following reference: Huang, X. and Madan, A. (1999) CAP3: A DNA sequence assembly program.

https://doua.prabi.fr/software/cap3

Abhijeet Singh

Simple answer - "YOU CAN NOT DO IT", based on the information and the way this question is being asked.

Complicated answer - much more information would be required to answer if or whether you can do it and what is you data and what could be the strategy.