We are in process to identify the best suitable antagonistic bacteria for management of Sclerotinia sclerotiorum and Alternaria brassicae infecting oilseed Brassica. There are several methods to observe their growth inhibition but I would like to know the easy procee. First we put bacteria in meddle of the petri plate as zic zac line and fungus in centre of the half portion. Fungus will grow even over the bacterial growth. Than what will be the measurement of inhibition???
You should observe an inhibition in the fungus growth halo when it encounters the secondary metabolites produced by the bacteria.
Comparing with the control, just with the fungus growing on the plaque, you will be able to suggest the level of this inhibition.
Dear, Meena,
You must grow the bacteria on Nutrient Agar medium and grow the fungus on PDA medium. Then you must take four discs from bacteria which grow on the Nutrient Agar and put them around the plate which contains PDA and incubate it under 28 C for 24 hours. After that, you must put 5 mm diameter disc of the fungus growing on PDA taken from the margin of seven days old colony in the center of the plate and incubate again for about 5-7 days with left a control treatment without any bacteria. Finally, you must measure the inhibition zone which has no contacting between the fungus and the bacterium.
Dear sir,
To test the bacteria as biocontrol agent against the fungus one is dual culture method. Put the fungus in the middle of the petro dish and in the periphery keep bacterial disc or streak on the plate.
In the well diffusion method, bacterial extract or its compound tested with pouring in the well in PDA plate instead of putting bacterial disc.
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