I am using MC3T3E1 cells ( mouse preosteoblasts) for transfection with Xtreme9 reagent. 24 hrs after transfection I see lot of cell death. Is there a way to minimise it?
You may reduced the DNA quantity to be transfected. High quantities of DNA can also induce cell death. If you use FBS during transfection, reduce it or avoid it (depends upon cell type).