What are the social factors that could affect teacher's behavioral intention to use online learning?

What are the social conditions that may influence one's accessibility to digital content?

30 July 2020 3,539 2 View

How to generate a homodimer from a monomer in PyMol or other software?

I performed tertiary structure prediction of my enzyme in Modeller. However, it is predicted to be a homodimer. How can I obtain a homodimer from the monomer obtained by prediction in a reliable way?

07 April 2020 5,264 2 View

Is it necessary to go through an IRB if you want to conduct research independently (no affiliation with a university or organization)?

This question is regarding researchers who are interested in conducting research after grad school but are not affiliated with an organization or university.

23 June 2019 3,779 4 View

Can a PAP pen be used to prevent live cells growing outside a desired border?

I am using a flea cell flow system and need a hydrophobic border but sadly for the slides I am using for imaging they don't have the Teflon border. I was wondering if a PAP pen could be just as...

18 March 2019 202 2 View

How do you modify in-text citations on Mendeley so that they are organised chronologically instead of alphabetically?

Hello, I am using Mendeley as my reference manager. I currently have a merged in-text citation: (Atterbury et al., 2007; Ramesh et al., 1999; Smith & Huggins, 2009) I want it to be...

07 March 2019 393 7 View

Where can I find out where to buy the anti-PSA antibody used in the clinic?

I'm testing a new anti-PSA antibody, and I want to compare it to the one used at the moment in the clinic to detect prostate cancer. Does anyone know how to find out where this antibody comes...

21 January 2019 856 1 View

Can anyone identify the species of this mold ?

I'm a food science student that likes to try out with different recipes from time to time. So I have made a really odd wine reduction + plain yogurt panna cotta that taste awfully odd (and super...

31 October 2018 563 4 View

Which correction for type 1 error due to multiple correlations?

I'm trying to find a test to correct for type 1 error due to multiple correlations (15 variables). Although Bonferroni is generally considered too conservative, I'm having trouble finding any...

21 February 2016 1,463 10 View

How do I extract DNA from lactobacilli?

21 September 2015 9,866 5 View

Can I boil bacteria to release DNA over extracting the DNA?

I want to use DNA for RAPD PCR, i have only ever used boilates for PCR, but would it be better to extract the DNA using a kit?

07 June 2015 2,559 14 View

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10?

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10? Since, the growth curve from day 2 to 10 won't be like the "Logistic curve" it might not follow logistic...

03 March 2021 1,401 3 View

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02 March 2021 3,060 3 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

Do you need the CMV enhancer before the CMV promotor for a proper function of CMV on the gene that follows?

I'm a student and I have to produce a cell line with a knockin for the NRF2 gene with GFP. I have to put a promotor in front of the GFP because the gene will be too far away from the promotor of...

28 February 2021 7,127 2 View

What could be some reasons why unstimulated monocytes are getting activated?

Im doing PBMC isolation -> CD14+ enrichment using magnetic beads -> stimulation setup. My negative control is just cells in cRPMI but they seem to get activated over and over again.

28 February 2021 7,883 3 View

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28 February 2021 9,936 2 View

When is it unnecessary to change organisational culture?

As a former management practitioner, I recall a generally accepted view that changing organisational culture is difficult, so you should only attempt it if the organisational change that you are...

27 February 2021 2,595 8 View