Impact of Attachment Theory on Addiction?

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How to use a dry ice bath to flash freeze plant samples?

I am out of LN, and I have no choice but to actually just use a dry ice bath to freeze my samples because I need to transport my leaf samples. For context I will be performing RNA extraction of...

14 May 2024 749 1 View

What statistical test should I use and how should I present my data?

Im working on my dissertation and ive decided to find out what are the most successful factors that go onto a reintroduction project for my chosen species. for example I collected factors such as;...

27 March 2024 9,649 3 View

Help with Enzyme Kinetic Analysis - Octet HT 11.1 or Sigma Plot?

I need help analyzing enzyme kinetic data. I have data from the Octet K2 system. In my experiment, I load the sensor with our protein of interest (6XHis tag on my recombinant protein to...

20 March 2024 6,373 3 View

Can someone help me finalize my research question for my Thesis ?

Hello everyone ! I am a master student with a specialization in export management and I really need your help regarding the finalization of my research question. I read a couple of articles and...

23 February 2024 7,334 1 View

How to avoid the DNA degradation in dilutions ?

I have isolated good quality DNA from plant using CTAB method. when i diluted these DNA (20 ng/ul) the DNA get degraded. I have check the diluted DNA on 1% gel but i dont seen the DNA.

13 December 2023 1,807 1 View

Can I use a visible light to UV light converter to transform the visible light for a UV photosensor?

Hi, I'm working on designing a camera for a Venus Rover, and I've stumbled upon a UV photodiode that can handle temperatures up to a whopping 550 degrees Celsius. The only catch is, it's built...

13 November 2023 5,699 1 View

Who has Gamification in Primary or Elementary Research?

I need someone who can at least share with me his/her research about gamification in Primary or in Elementary Education. Thanks

27 September 2023 4,010 1 View

ITS2 secondary structure of Rhodotorula (basidiomycota)?

Dear all, I'm trying to do the ITS2 secondary structure of a Rhodotorula (basidiomycota). I predicted the ITS2 sequence using ITSx and also ITS2database for comparison. I tried to fold the...

26 March 2023 5,102 0 View

Whom would you choose? a relative of a good and trusted friend or an applicant who is better?

A relative of a good friend and trusted worker is applying for a job in your school. Another applicant for the same job is better. Whom would you choose if you were the personnel manager.

24 March 2023 7,370 3 View

How to measure optical density of bacterial cells in petri dish?

For my experiment, I am trying to observe the growth of two gut microbes against in vitro agar substances which represent dietary fibre (turmeric, golden seal, and citric acid) and fats (palmitic...

07 February 2023 5,585 6 View

Are there any commercially available Donkey anti-Alpaca secondary antibodies?

Are there any fluorescently labeled anti-Alpaca secondary antibodies raised in Donkey? So far I have only been able to find anti-Alpaca secondaries raised in Goat. Or is this not possible due to...

04 August 2024 4,255 1 View

Which solvent is better to dissolve with secondary metabolites extracted from fungi?

I work on MCF7 cell cell for anticaner purpose and I wa to do drug preperation the drug ( secondary metabolites extracted from Aspergillus) My question which solvent is better with these secodary...

03 August 2024 4,725 2 View

What should I do in this situation ?

I'm an undergraduate university student conducting research on the effects of internet use on depression and anxiety among undergraduate Ghanaian university students: Mediation role of Internet...

31 July 2024 2,979 3 View

Questions of antibodies selection?

Hi there, I'd like to use immunocytochemistry to determine a surface protein expression on mouse cell. The primary antibody I have is mouse anti mouse, and the secondary antibody I have is goat...

15 July 2024 6,008 5 View

Does plant based medicine have less side effects?

I was reading papers on plant secondary metabolites and their applications and many of the papers/books I read, have the point that plant-based medicines which are the result of application of...

08 July 2024 9,935 10 View

Can I use BSA for a Histological Blocking Solution?

Hi all, I would like to know if it is possible to use Bovine Serum Albumin (BSA) in my blocking solution for immunofluorescence muscle fiber typing. The primary antibodies' host is mouse. The...

07 July 2024 7,967 3 View

How can I avoid uneven IHC staining on free floating tissue from frozen human CNS sections (cryostat 50um thickness)?

Hello, I've been doing free floating IHC using a VAChT antibody with a 1:1k dilution (139 103 - Synaptic Systems). However, there has been a struggle to keep an even stain with my samples of...

06 July 2024 4,521 2 View

How is the preparation to obtain secondary metabolic extracts?

Hi everyone! I am working with bacteria and fungal collected from marine sediments. I would to know the methodology since the preparation of biomass until the extraction and stored of secondary...

03 July 2024 6,177 3 View

Can the MDFT mental health care model help reduce drug addiction?

This question might come up in my research on "Frustration, Fantasy, and Drug Addiction," where I'm also trying to figure out how to overcome mental health issues without turning to drugs.

30 June 2024 5,936 0 View

Nutrition map: What does it mean for you?

Unluckily, just similar to an expensive car, your brain can be damaged if you ingest anything other than premium fuel.

23 June 2024 2,287 7 View