What are the social factors that could affect teacher's behavioral intention to use online learning?

What are the social conditions that may influence one's accessibility to digital content?

30 July 2020 3,539 2 View

How to generate a homodimer from a monomer in PyMol or other software?

I performed tertiary structure prediction of my enzyme in Modeller. However, it is predicted to be a homodimer. How can I obtain a homodimer from the monomer obtained by prediction in a reliable way?

07 April 2020 5,264 2 View

Is it necessary to go through an IRB if you want to conduct research independently (no affiliation with a university or organization)?

This question is regarding researchers who are interested in conducting research after grad school but are not affiliated with an organization or university.

23 June 2019 3,779 4 View

Can a PAP pen be used to prevent live cells growing outside a desired border?

I am using a flea cell flow system and need a hydrophobic border but sadly for the slides I am using for imaging they don't have the Teflon border. I was wondering if a PAP pen could be just as...

18 March 2019 202 2 View

How do you modify in-text citations on Mendeley so that they are organised chronologically instead of alphabetically?

Hello, I am using Mendeley as my reference manager. I currently have a merged in-text citation: (Atterbury et al., 2007; Ramesh et al., 1999; Smith & Huggins, 2009) I want it to be...

07 March 2019 393 7 View

Where can I find out where to buy the anti-PSA antibody used in the clinic?

I'm testing a new anti-PSA antibody, and I want to compare it to the one used at the moment in the clinic to detect prostate cancer. Does anyone know how to find out where this antibody comes...

21 January 2019 856 1 View

Can anyone identify the species of this mold ?

I'm a food science student that likes to try out with different recipes from time to time. So I have made a really odd wine reduction + plain yogurt panna cotta that taste awfully odd (and super...

31 October 2018 563 4 View

Which correction for type 1 error due to multiple correlations?

I'm trying to find a test to correct for type 1 error due to multiple correlations (15 variables). Although Bonferroni is generally considered too conservative, I'm having trouble finding any...

21 February 2016 1,463 10 View

How do I extract DNA from lactobacilli?

21 September 2015 9,866 5 View

Can I boil bacteria to release DNA over extracting the DNA?

I want to use DNA for RAPD PCR, i have only ever used boilates for PCR, but would it be better to extract the DNA using a kit?

07 June 2015 2,559 14 View

Is there a way to grow biofilm in lab to mimic biofilms in showers and baths? Also, how can I measure it?

Hello, I am new to biofilm research and have a question about growing and measuring biofilms. We want to research the effect of a type of enzymes in breaking down biofilms in showers, baths, hot...

14 February 2021 3,522 3 View

(western blot) can methanol damage my antibody signal?

I accidentally rinsed with methanol after adding my primary antibody to my membrane. I noticed after ~10 seconds and immediately replaced it with PBST (3x15 min washes). Will my antibody signal be...

12 February 2021 5,824 3 View

Is secondary metabolite production more at vegetative growth stage or during reproductive stage?

Hi, I am working on the effect of heavy metals on a medicinal plant by pot experimentation. Analyzing the effect of heavy metals on secondary metabolites is one of the parameters. I will be...

11 February 2021 6,683 6 View

How can I measure speed of integration of an acquisition with secondary data?

I am currently faced with the challenge to measure integration speed of an acquisition or a merger with secondary data. Usually companies do not communicate once a target has been integrated...

27 January 2021 9,741 11 View

Can i re-incubate a WB membrane with a higher solution of the previously used 1ry ab?

I've incubated my western-blot membrane with both primary and secondary antibodies and i just realized at the end that i've used a lower concentration of my primary antibody for my loading control...

27 January 2021 1,034 3 View

How to prepare secondary antibody from lyophilized antibody?

So I'm new to lab research and learning Western Blots these days. I ordered my secondary antibody and it arrived in a powdered form. Here is the...

27 January 2021 5,402 3 View

Can you do Ponceau staining after loading control for Western Blot?

Hello everyone! I forgot to take a picture of my Ponceau stain prior to starting my first round of primary and secondary antibodies. I'm currently doing my loading control now and was wondering...

25 January 2021 757 3 View

Is there a paper on the development of the SQFM(AA), the Shorter questionnaire for family members affected by addiction?

I can't find anything on the validation of this tool. It measures stress/strain/coping/support for family members of substance users (or those addicted to gambling).

23 January 2021 1,463 4 View

Is there no general protocol for vaccination study?

Hi, I searched vaccination study to know which MLD50 is suitable for influenza virus study in pubmed. But why every vaccination and challenge conditions are different? Is there no guideline or...

16 January 2021 7,984 3 View

What sampling strategy is utilising secondary data, reducing survey population into sub-groups?

I am writing a research proposal, using a universities' convenience survey data that they have conducted. In order to answer my research question, I will need to look at two specific generational...

09 January 2021 3,220 4 View