Dear all,

I'm trying to do the ITS2 secondary structure of a Rhodotorula (basidiomycota). I predicted the ITS2 sequence using ITSx and also ITS2database for comparison. I tried to fold the sequence using ITS2database, RNA webserver, mfold or contrafold. I keep getting a weird strucutre even if I add the stem sequence (5.8S and 28S). I can't say if my strucuture is correct or not because the only structure that I could find online are for ascomycota (3 helixes). Does anyone know how the ITS2 secondary structure of Basidiomycota such as Rhodotorula looks like ? (helix number ? length ? and form ?). Thank you in advance for your help.