Hello,
I am currently looking for a protocol on how to quantitate complement receptor 1 molecules PER red blood cell using flow cytometry. I am aware that in order to do this, a standard curve has to be generated first and from then if using samples will be able to use this to semi-quantify how many there are in my samples.
Any ideas?
Thanks.
Hello
Take a look
Article A flow cytometric assay for measuring complement receptor 1 ...
Hi Saleh Alkarim ,
Thank you for this. Unfortunately both techniques and this is what I've seen in most papers actually, uses radioligands (25I mAb) which I am trying to avoid to use. Therefore, I am trying to find any alternatives if possible.
Hi April,
If I were doing this my approach would be to use multiple dilutions of a known number of control RBCs and stain them for CR1 and look at the median or geometric mean. It is the same principle as using radiolabelled, measurement of a mean. You can work backwards from the mean or median to figure out roughly how many ab have bound to the RBC. The attached file hopefully gives you enough to know in what direction to look.
Good luck
NM
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