April Baral

4 Questions 3 Answers 0 Followers

Questions related from April Baral

Why do we use sheep and rabbit erythrocytes for classical and alternative pathway haemolysis assay, respectively?

Hello. I am currently running haemolysis assays to measure inhibitory effect of some complement regulatory proteins. The assay involves complement activation by either the classical pathway (CP)...

03 May 2021 8,229 0 View

How to use biotinylated primary antibody; streptavidin APC secondary antibody in compensation beads to get positive population?

Hello, I am currently setting up my compensation matrix in flow cytometry and to do this I am using compensation beads (versacomp antibody capture beads) to get negative and positive population...

14 February 2019 2,447 4 View

Ideal centrifuge speed for isolated leukocytes prior to cell pellet freezing?

Hello, I am currently optimising my protocol in terms of isolating leukocytes from a method by just collecting the "buffy coat" after spinning whole blood at 3000g 20mins. This is the protocol...

11 February 2019 768 1 View

How to measure complement receptor 1 per erythrocyte using flow cytometry?

Hello, I am currently looking for a protocol on how to quantitate complement receptor 1 molecules PER red blood cell using flow cytometry. I am aware that in order to do this, a standard curve has...

06 January 2019 5,492 3 View