Minimal protein concentration from BCA can be detected using western blot?

hello, i extracted protein from PBMC's and after applying BCA assay on 2 samples the protein concentration was 400 ug/ml which means 0.4 ug/ul so does western blot work with these quantities? any...

24 December 2020 6,521 3 View

Is glucose sensor has another application or target rather than diabetes ?

I need a chapter or paper discussing list of application of glucose sensors , can anyone help ? thanks

02 June 2020 6,635 1 View

What is the fastest and most efficient method to wash acidic activated carbon?

The carbon was activated using phososhoric acid.

03 March 2020 5,256 4 View

Why stats tools package (excel) not working?

I downloaded the excel stats tools package, attempt to run it after filling the suitable required info and press the bottom. Unfortunatelly it does not work. I enabled the macro in my PC, still...

24 June 2018 6,663 4 View

I have undiscriminant validity between two constructs. How to settle this?

I discovered the undiscriminant validity between the two constructs when I observed that the values of MSV are higher than AVE for each factor; also the factor analysis _reduction test (validity...

23 May 2018 5,714 3 View

Difficulty with western wet transfer

Does anyone have any experience with wet transfer? When I use a the transfer buffer to wet transfer my blot, the marker bands 'smudge' across the membrane. They do not remain sharp, and therfore...

16 July 2012 2,665 21 View

Private or public agricultural advisory systems

Do you believe that a private advisory services can paly a good role in agricultural extension?

01 January 1970 3,042 32 View

Teaching and Training

What is the difference between the teaching and the training?

01 January 1970 1,959 22 View

Is there any test to determine the cardiovascular risk of patients with obesity? from the perspective of obesity?

We found tests to determine cardiovascular risk taking into account lipid profile, blood pressure, age, history of diabetes, other laboratories. But when weight is involved, there is a paradox. I...

02 March 2021 6,036 1 View

No title

02 March 2021 3,060 3 View

What SPSS analysis should I run with one IV (with two levels) and two DVs?

Hi, I am trying to figure out which test would be most appropriate to run for the following question: Is there a difference in task blood pressure between smokers and non-smokers? There are two...

01 March 2021 8,235 2 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

Do you need the CMV enhancer before the CMV promotor for a proper function of CMV on the gene that follows?

I'm a student and I have to produce a cell line with a knockin for the NRF2 gene with GFP. I have to put a promotor in front of the GFP because the gene will be too far away from the promotor of...

28 February 2021 7,127 2 View

What could be some reasons why unstimulated monocytes are getting activated?

Im doing PBMC isolation -> CD14+ enrichment using magnetic beads -> stimulation setup. My negative control is just cells in cRPMI but they seem to get activated over and over again.

28 February 2021 7,883 3 View

No title

28 February 2021 9,936 2 View