If you have no budged problem, I advise mirvana kit.

The miRNeasy kit and the mirvana kit have both worked well for me.

hi Ayah Al Qaryoute

-- For microRNA work, go for Qiagen products, their all microRNA research products are the best in the market.

-- You can isolate total RNA from your samples using TriZol method. Then, you can use the Qiagen miscript-II-RT kit for cDNA synthesis. This kit will convert all miRNA present in the sample into miRNA-specific cDNA. Now, you can analyze your miRNA of interest by SYBr qRT-PCR using miRNA primers.

-- Even you can buy specific miRNA isolation kit and isolate miRNA. But ultimately cDNA synthesis will matter for further qRT-PCR.

Keep this point in a note that

-> All miRNA cDNA synthesis kit not works with SYBr, many only works with Taqman. So before buying check its requirements.

-> Prefer to use a pre-designed miRNA primer.

-> Use at least 3 endogenous control.

Now, miRNA primer has universal sequences. you will find the stem-loop & mature miRNA sequence in the miRDB database. Well, as I said, prefer predesigned primers of your interested miRNA from any standard company. Nowadays, Companies do a slight modification or say a technology, which enhances stability and avoids unwanted binding. Qiagen has LNA tech. based miRNA primers, those are readymade, pre-mix (FP+RP), available for most of the miRNAs. Just dissolve and use 1ul for per rxn.

Regards

Saurabh