14 September 2021 4 6K Report

I’m doing a Ph.D. to design tools to make it easier for people to review the literature. Can you help me make it better? Please answer this questionnaire which will take around 10 minutes only, and you can choose to enter a prize draw to win one of five Amazon shopping vouchers, each worth £50: https://strathsci.qualtrics.com/jfe/form/SV_djmpf8NgDR8EjH0

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I am Ayah Soufan, a 3rd-year Ph.D. researcher at Strathclyde University interested in designing systems to help scholars conduct literature reviews. If you are a Master's student in Computer...

13 April 2023 9,454 1 View

How to handle large seurat objects (5GB+) in R?

I have integrated 11 seurat objects that I need to merge before downstream analysis, I combined the first 4 (2.6GB total) and saved them as an rds object just fine but every time I combine the...

22 November 2022 1,785 3 View

How to map Affymetrix probes from microarray data into gene symbols or IDs for Clariom_S_Human annotation?

I have microarray data from Affymetrix Clariom S Assay, Human chips, I read the .cel files but when I do the RMA normalization I get the following error: " Error in getCdfInfo(object) : Could...

19 September 2022 5,570 1 View

How to perform a Differentially methylated regions analysis (DMRs/DMC) using GenomicRationSet?

I want to find the differentially methylated regions between two groups of samples, I have a dataset containing the beta values for each sample mapped to each gene and the corresponding grouping...

24 July 2022 8,869 0 View

How to avoid having multiple differenet Tm peaks in the qrt-PCR Melt Curve when having 3 different genes ?

Hi, I have 3 genes (1st is my Gene of interest and two house keeping genes) each gene will have a forward primer , a reverse primer and a different Tm for each primer. 1-When doing the cDNA...

03 May 2021 3,739 4 View

Hi all ,What is the best protocol and reagents to extract microRNA from platelets for qrt-PCR ?

am looking for a protocol to extract microRNA from platelets where I can use for qrt-PCR any recommendations please?

22 April 2021 4,733 3 View

Minimal protein concentration from BCA can be detected using western blot?

hello, i extracted protein from PBMC's and after applying BCA assay on 2 samples the protein concentration was 400 ug/ml which means 0.4 ug/ul so does western blot work with these quantities? any...

24 December 2020 6,874 3 View

How to ensure right PBMC's count?

hello there! I am isolating PBMC's from heparinized blood using density gradient method and after having the pelette we count cells using a hemocytometer by taking 5 ul cells with 10 ul trypan...

19 October 2020 4,341 19 View

How to measure cell suspension concentration or wight?

hello there! I'm going to use M-per protein extraction kit for PBMC'S , the protocol recommended to add 1 ml of the reagent for each 100 mg or 100 μL cell suspension so how could we measure cell...

02 October 2020 5,621 2 View

How could we apply whole blood sonication?

hello there! i want to apply ELISA on a whole blood samples but in the leaflet sheet they mentioned that whole blood samples need to be sonicated so how could i know the best protocol for...

17 August 2020 7,391 6 View

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