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Questions related from Ayah Alkhaldi
hello, i extracted protein from PBMC's and after applying BCA assay on 2 samples the protein concentration was 400 ug/ml which means 0.4 ug/ul so does western blot work with these quantities? any...
25 December 2020 6,788 3 View
hello there! I am isolating PBMC's from heparinized blood using density gradient method and after having the pelette we count cells using a hemocytometer by taking 5 ul cells with 10 ul trypan...
20 October 2020 4,325 19 View
hello there! I'm going to use M-per protein extraction kit for PBMC'S , the protocol recommended to add 1 ml of the reagent for each 100 mg or 100 μL cell suspension so how could we measure cell...
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hello there! i want to apply ELISA on a whole blood samples but in the leaflet sheet they mentioned that whole blood samples need to be sonicated so how could i know the best protocol for...
18 August 2020 7,367 6 View
hello! we will extract peripheral mononuclear cells, freeze them, and apply ELISA so which anticoagulant is the best for blood collection in this case?
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