Ayah Alkhaldi

3 Questions 9 Answers 0 Followers

Questions related from Ayah Alkhaldi

Minimal protein concentration from BCA can be detected using western blot?

hello, i extracted protein from PBMC's and after applying BCA assay on 2 samples the protein concentration was 400 ug/ml which means 0.4 ug/ul so does western blot work with these quantities? any...

25 December 2020 6,757 3 View

How to ensure right PBMC's count?

hello there! I am isolating PBMC's from heparinized blood using density gradient method and after having the pelette we count cells using a hemocytometer by taking 5 ul cells with 10 ul trypan...

20 October 2020 4,316 19 View

Which type of blood tubes is the best for PBMCs extraction?

hello! we will extract peripheral mononuclear cells, freeze them, and apply ELISA so which anticoagulant is the best for blood collection in this case?

09 November 2019 2,900 6 View