Ayah Alkhaldi

6 Questions 16 Answers 0 Followers

Questions related from Ayah Alkhaldi

Minimal protein concentration from BCA can be detected using western blot?

hello, i extracted protein from PBMC's and after applying BCA assay on 2 samples the protein concentration was 400 ug/ml which means 0.4 ug/ul so does western blot work with these quantities? any...

25 December 2020 6,973 3 View

What is the ideal volume of M-PER should be added to cell pellet?

Hello! I am using M-PER protein extraction kit to extract whole protein from PBMC's but till now i have a problem with the appropriate volume of the reagent that should be added so did anyone use...

26 October 2020 8,276 5 View

How to ensure right PBMC's count?

hello there! I am isolating PBMC's from heparinized blood using density gradient method and after having the pelette we count cells using a hemocytometer by taking 5 ul cells with 10 ul trypan...

20 October 2020 4,363 19 View

How to measure cell suspension concentration or wight?

hello there! I'm going to use M-per protein extraction kit for PBMC'S , the protocol recommended to add 1 ml of the reagent for each 100 mg or 100 μL cell suspension so how could we measure cell...

03 October 2020 5,641 2 View

How could we apply whole blood sonication?

hello there! i want to apply ELISA on a whole blood samples but in the leaflet sheet they mentioned that whole blood samples need to be sonicated so how could i know the best protocol for...

18 August 2020 7,409 6 View

Which type of blood tubes is the best for PBMCs extraction?

hello! we will extract peripheral mononuclear cells, freeze them, and apply ELISA so which anticoagulant is the best for blood collection in this case?

09 November 2019 2,953 6 View