I personally would not pipette 1µl with a multichannel pipette but rather dilute the drug in a way that the desired final concentration is reached by adding at least 3µl.

Sabine Strehl drugs are diluted in DMSO. DMSO is toxic to cells at high concentration.

To ensure that you add exactly 1 µl of a drug solution to your cancer cell culture using a multichannel pipette, follow these steps:

Remember to handle the multichannel pipette carefully to maintain accuracy throughout the experiment. Additionally, always use fresh, sterile pipette tips for each sample to avoid cross-contamination.

If you require the most precise and accurate volume measurements, consider using a calibrated automatic pipetting system or a liquid handling robot, as these instruments can provide higher accuracy and reproducibility compared to manual pipetting.

best ..

I agree with the Sabine. Dilute your drugs in the cell culture media to increase the volume that would serve as a working solution. Then you can use multichannel to pipette out larger volume to add to your cells.

Abdul Mannan at a very low concentration of drugs, the dilution is not linear.

using an electronic pipette can somewhat increase accuracy, but I agree with the above comments that some kind of dilution should be applied.

https://lab.plygenind.com/manual-vs-electronic-pipette-differences