Abdul Mannan

11 Questions 13 Answers 0 Followers

Questions related from Abdul Mannan

Why MV4-11 cells transduced with lentivirus containing GFP and puromycine are dying in puromycine?

Hi Everyone, I have transduced MV4-11 with the lentiviral particles of HuSH shRNA GFP Lenti Cloning Vector (pGFP-C-shLenti Vector) from Origene. This vector contains both GFP (driven by CMV...

17 January 2022 9,930 5 View

Does anyone has experience of doing MCF7 tamoxifen cytotoxicity assay?

I am treating MCF7 cells for 96hrs and my protocol is as follow 1-Day 1- Seed cells @ 2.5x10^3 cells/100ul per well in 96 well plate in 10% FBS containing DMEM complete media 2- Day 2- Drug...

01 August 2017 2,733 4 View

If ZR-751 is a luminal A or B cell line?

We are working on breast cancer with the main focus on luminal B tumours. We have been using BT474, ZR751 and ZR7530 cell lines as a model for luminal B breast tumour, but recently I came across...

25 January 2017 4,154 2 View

Can anyone help me with the Soft Agar Assay?

We are trying to do soft agar assay in BT474 (luminal B) breast cancer cells. I tried couple of times, but could not get even a single colony. Last time I even tried using MCF10A-Ras cells but...

11 March 2016 2,279 9 View

Can anyone help me with a Transwell matrigel invasion assay and wound healing assay on BT474 Breast cancer cells?

I am doing Transwell matrigel Assay on BT474 cell lines as follow To coat trasnwell inserts (8um pore) I added 75 ul of 2mg/ml matrigel in serum free media and incubated in the incubator for about...

07 December 2015 3,831 6 View

Any software to count gamma H2AX S139 and p-ATM Foci in DNA damaged cells?

I have done some DNA damage experiments and performed immunoflourescence. My boss asked me to count gamma H2AX and p-ATM foci in 100-200 cells. I reckon to count these manually is quite tough...

30 August 2015 6,476 5 View

Any advice on MCF-7 Soft Agar Assay colony formation and crystal staining?

Hi, I did soft agar assay on MCF-7 cells. I used 0.7% and 0.5% for plate 1 and for plate 2 0.5 and 0.35% of agarose for bottom and upper layers respectively. 5000 cells were seeded in each well....

27 April 2015 214 1 View

Can anyone help with Western Blot analysis?

I am doing western blot and the problem that I encounter is that the target protein and housekeeping proteins' molecular weight is close enough to cut the gel to probe on separate membranes....

20 August 2014 6,397 8 View

Can anyone help with siRNA transfection?

I am using K562 cell line. Recently, I tried to transfect these cells with siRNA bought from qiagen (flexitube siRNA). For optimization I used forward transfection method in twelve well plate. We...

15 May 2014 4,827 4 View

Anyone familiar with imatininb stock solution storage and stability?

We are planning to treat CML induced mice with aqueous solution of Imatininb. My question is, how long and at what conditions (temperature and humidity) can we store aqueous solution of Imatininb ?

18 February 2014 1,923 1 View

Can anyone help with Imatinib stock solution sterilization?

We are planning to treat mouse model of CML with Imatininb. We want to prepare a water base Imatininb stock solution and my question is: How do we sterilize that solution? Should we prepare by...

17 February 2014 4,168 5 View