I've designed a recombinant protein made of 5 parts from 5 different proteins. And I have the sequence of the whole recombinant protein I designed. It's around 2kb.
Now the problem is where/how to get those DNA pieces and assemble them together? Will it be easier to directly ask a company to synthesize the cDNA?? I need a fastest and easiest way. Thanks!!
Dear Wip
if you do have stringent budget restriction the fastest way is certanily buy a syntetic gene.
you can buy it from different companies as Genescript, Gene art (https://www.thermofisher.com/it/en/home/life-science/cloning/gene-synthesis/geneart-gene-synthesis.html) , DNA 2.0 (https://www.atum.bio/) and probavly many others.
Check which is the best in terms of cost and shipment timelines in your country.
good luck
Manuele
The best option to get your recombiat protein in short time is to get it synthesized, I have used genescript for this purpose and you can get your gene cloned into the plasmid (If you use one of their archived Vector it would be very cheap)
https://www.genscript.com/gene-synthesis-gensmart-ai-ordering.html?src=service
Hope it helps
https://www.genscript.com/gene-synthesis-gensmart-ai-ordering.html?src=service
Hi Suipwksiow,
I just saw your post about your recombinant protein needs. We at Biomatik got you! We will take on your project from the very beginning (Gene synthesis) and will deliver you the purified protein and also the vector containing the gene insert if needed.
One thing I would like to point out is that we offer a risk-free guarantee for most proteins (95% success rate!
To help us evaluate your project properly, please submit your inquiry by using our online protein quote request form: https://www.biomatik.com/services/recombinant-protein-production.html
We look forward to hearing from you and working together on your next project.
Best regards,
Theresa
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