For HDAC inhibitors, which HDAC member(1/2/3/4...) is inhibit by it?

More Suipwksiow Wjkp's questions See All

How to validate anti-human-immune-checkpoint in mouse model (in vivo)?

The antibody is against human protein, but with mouse IgG (as it is generated in mouse). Before totally humanizing it (which takes a lot of money and time), I want to validate its function in...

20 April 2022 9,928 0 View

Can FDA-approved drugs be used in other disease setting?

Hi Everyone, I'm exploring the anti-tumor effect of some drugs. I'd like to test some FDA-approved drugs which are not used to treat cancer. My question is, if there is any anti-tumor effect for...

14 December 2020 1,630 1 View

How to do proper human T cell proliferation assay?

Hi Everyone, I did numerous human T cell CFSE proliferation assays, but the peaks never shift. I put CD3/CD28 and IL2, and I can see that T cells did proliferate by my eyes (as there was increase...

08 December 2020 9,238 3 View

How to know if my protein forms a dimer or not?

Hi everyone, The protein I studied is a membrane protein. Some study indicates that there is possibility that this protein can form a dimer. I did standard western blot for this protein and my...

20 October 2020 7,150 7 View

What assay should I do to confirm membrane protein-protein binding?

Hi all, My hypothesis is that CD99 binds to CD88 (two membrane proteins). My colleagues suggested me to do co-IP, but I don't have good western blot antibody against one of the protein. However,...

14 October 2020 9,377 6 View

How to ensure a marker antibody won't activate T cells and affect the result?

I'm recently buying some anti-CD3 and anti-CD28 to activate T cells. I also notice some of this clones have been conjugated with fluorescence for the purpose of labeling T cells for flow...

06 October 2019 7,384 3 View

To synthesize chimeric receptor, should I used real gene sequence or artificial sequence?

I'm synthesizing a chimeric antigen receptor (CAR), and I have the whole sequence of the amino acid. Now the problem is, to ask company to synthesize the DNA of the CAR, should I send real gene...

06 February 2019 1,641 1 View

Why my gene in single-cell sequencing showed zero value?

I have many single-cell sequencing data. I checked my gene of interest in these data. However, all of these single-cell sequencing data showed that my gene has no expression, no matter in immune...

03 January 2019 1,876 3 View

Why can't I detect the surface protein by western blot?

I was doing an over-expression experiment of a gene for a year. It is a surface protein. The biggest trouble is that I can never detect the gene overexpression by western blot. Interestingly, when...

29 November 2018 6,457 3 View

For CRISPR, transfection or transduction?

For CRISPR, transfection or transduction? The CRISPR vectors I bought is lenti vector, so I can either do transfection or transduction. But which is better??

13 March 2018 4,611 3 View

Can i use the protease inhibitors during cell membrane vesicle preparation ？?

I am currently working on a project that involves extracting cell membranes, for which we disrupt the cells using sonication. During the initial extraction process, we add protease inhibitors to...

30 July 2024 7,077 1 View

How do you determine the Ki of an inhibitor for an enzyme which also undergoes substrate inhibition?

There are three factors involved in the activity, the substrate acting as a substrate, the substrate acting as an inhibitor and another inhibitor for which you want to measure the Ki.

08 July 2024 4,988 1 View

?como elimino articulos que no son propios?

eliminoar articulos que son de tu autoria

05 July 2024 2,107 1 View

In what ways do economic factors influence family dynamics and relationships?

Economic factors influence family dynamics.

02 July 2024 6,235 0 View

Name of my daughter?

karma

20 June 2024 6,189 1 View

¿ Los paises en vias de desarrollo son mas abiertos frente a las politicas migratorias?

Es posible observar que mientras que paises como colombia con altos indices de pobreza y desigualdad, abrieron sus fronteras a millones de migrantes venezolanos, por otro lado algunos paises de la...

16 June 2024 236 2 View

How to incease the yiald of recombinant protein expression ?

Hello esteemed scientists, I am endeavoring to increase the yield of my recombinant protein. It is a 120 kDa protein tagged with MBP and features three intrinsically disordered regions with pI...

12 June 2024 9,616 8 View

Can you explain the potential toxicity and overdosemanagement of commonly used antiplatelet drugs?

Antiplatelet Therapy: Mechanisms, Indications, Administration Can you explain the potential toxicity and overdosemanagement of commonly used antiplatelet drugs?

05 June 2024 6,447 1 View

How to prepare 0.28 U/ml working solution of Acetylcholinesterase inhibition assay for anti-Alzheimer activity?

I have purchased Acetylcholiesterase from Electrophus electricus (electric eel), C3389-2KU, following details are written on the enzyme vial. Type-VI S lyophilized powder 200-1000 Units/mg...

31 May 2024 6,444 1 View

Can I delete my duplicated profile asap?

Hello, I duplicated my research account by mistake recently. The recent one shows 2 research publications. I would like to delete it immediately but I don't know how. Can you assist please? I...

28 May 2024 905 0 View

Craig Silver

Depends upon the inhibitor you use. https://en.wikipedia.org/wiki/Histone_deacetylase_inhibitor#HDI_classification

Sk Abdul Amin

Dear Suipwksiow Wjkp,

From the following articles (links attahced) you may have a broad about HDAC classes (with special emphasis on HDAC8).

https://goo.gl/X5w1u1

https://goo.gl/q6LyrX

Thanks,