Which fluorescence wavelength should I choose?

06 July 2018 6,266 3 View

Is it possible to dye two fluorescence at the same time ?

I'm preparing for immunofluorescence assay in live cell. Is it possible to dye two fluorescence at the same time ?

06 July 2018 8,714 2 View

How to treat GPNA drug in cancer cells?

Hi, everyone. I'm a master student, but I run into limitations about my study. How to treat GPNA drug in cancer cells? I treated GPNA drug in cancer cells via a leading journal, but almost all...

03 April 2018 4,732 2 View

Should you collect dead floating cells as well and harvesting live cells when performing the Trypan blue assay?

Should you collect dead floating cells as well and harvesting live cells when performing the Trypan blue assay?

02 March 2018 8,505 4 View

How to use drug working in high concentration ?

We have to dissolve GPNA (gamma-L-Glutamyl-p-nitroanilide; MW: 303.7) with proper solution. Datasheet notices this reagent is soluble in water (5 mg/ml), so we tried to dissolve this in water....

01 February 2018 3,172 2 View

How to experiment animal test of naturally regressed tumor or senescent xenograft in BALB/nu mouse?

We implanted specific tumor cells (10^7 cell)and after 3 days, I checked tumor (diameter : about 3 mm). However, from tumor injection to now, tumor size of all mouse was not changed . Is it...

11 December 2017 9,940 6 View

How to dissolve alpha tocopherol to use in cell culture?

I puchased alpha tocopherol (cat.258024) in SIGMA-ALDRICH, but This product is oily solution. How to dissolve alpha tocopherol to use in cell culture?

07 August 2017 2,348 3 View

What is preferred method among MDA assay and FACS using BODIPY dye in measurement of lipid peroxidation?

What is preferred method among MDA assay and FACS using BODIPY dye in measurement of lipid peroxidation? I want to know why, also.

04 May 2017 9,883 1 View

Does ROS or lipid ROS increase, if detached cell is treated by drug inducing ROS despite of originally growing cell to be adherent ?

04 May 2017 2,799 4 View

Why two peaks occur in dcfda assay through facs ?

Why two mountains occur in dcfda assay through facs ? my protocol is following 1. detached cell using TE and drug treatment in HBSS media. 2. after appointed time (1,2 h), dcfda staining during 30...

04 May 2017 6,487 3 View

Buffer to disrupt platelet alpha granules for PF4 quantification?

Hello! I want to quantify by ELISA the secreted (from platelets poor plasma) and the non-secreted (from platelet lysate) PF4 before and after TRAP stimulation. I will use the ELISA from R&D...

03 March 2021 1,499 2 View

Buffer Exchange using column packed with sephadex G-25 effect on protein concentration?

Hello, If i am doing a buffer exchange for an antibody of 1mg/mL, does the elution lose protein in the process of buffer exchange? For example, if i flow through 500 uL of 1mg/mL sample, and...

03 March 2021 6,299 3 View

How optimize the Size Exclusion Chromatography?

Hello! I'll do a size exclusion chromatography, but I only have an open column, and I'll perform the peptide extraction from yeast, using buffer lysis (sodium phosphate 50 mM/NaCl 30 mM/DNAse and...

01 March 2021 2,215 2 View

Why are my bands weird in this Agarose gel?

Can someone please give me some possible things that could go wrong? Here is my recipe: 0.5g Agarose 50 mL of TAE 1x 1 uL ethyl bromide. Gel was run at 100V for 1 hour. The buffer used is also TAE.

01 March 2021 9,952 3 View

What do I do with DLS peaks that are from the buffer?

I'm looking at the aggregation of my protein sample using DLS. Unfortunately, my buffer (20mM HEPES) also results in a set of peaks. These are at approximately 1 and 1000 d.nm. The lowest peak...

01 March 2021 9,015 2 View

What is the most suitable resuspension buffer for TCA/acetone precipitated protein?

I need to extract protein from fermented carbon sources for Bradford assay. Most researchers experiencing insolubility of pellet in resuspension buffer. Please assist me to select most suitable...

26 February 2021 7,129 3 View

What urea buffer can i use for biotagged protein extraction from streptavidin beads?

Hi all, I have been doing research on biotagged LDB1 proteins in Mel cells. I purify the proteins using M280 streptavidin beads. The yield is very low so I've been trying to optimize it by...

24 February 2021 5,749 3 View

Cloudy/high background western blot - can anyone help me?

Hi everyone! I try to detect VDR protein (~48 kDa) using Western Blot. After SDS-PAGE using 10% MP TGX Stain-Free Gel from BioRad I transferred the protein onto an metOH activated PVDF membrane...

23 February 2021 6,266 3 View

How to improve the buffer capacity of sea water?

Hello everyone I would like to improve the buffer capacity of seawater (pH ~8) in order to dissolve as much CO2 as possible without dropping the pH below 7.5 (a range between 7 - 7.5 is...

17 February 2021 2,797 2 View

Why does flow deformation occur at the test line and control line on nitrocellulose membranes (LFIA)?

I use 1% BSA in 10 mM phosphate buffer (pH 7.4) solution when striping my nitrocellulose membranes with antigens and antibodies. However, I always observe deformed flow when my sample or running...

16 February 2021 4,224 3 View