17 Questions 15 Answers 0 Followers
Questions related from Daiha Shin
Hi, My name's Daiha Shin. I have 5x protein sample buffer (not including DTT). I don't know that 'how to make DTT-included sample in WB with upper sample buffer? I have tried to find this...
07 July 2018 8,071 0 View
I'm implementing RPA assay using fluorescence microscope. Protocol of its assay mentions that "The red fluorescence of RPA is excited at λ exc.=543 nm and collected through a long-pass filter...
07 July 2018 6,363 3 View
I'm preparing for immunofluorescence assay in live cell. Is it possible to dye two fluorescence at the same time ?
07 July 2018 8,785 2 View
Hi, everyone. I'm a master student, but I run into limitations about my study. How to treat GPNA drug in cancer cells? I treated GPNA drug in cancer cells via a leading journal, but almost all...
04 April 2018 4,802 2 View
Should you collect dead floating cells as well and harvesting live cells when performing the Trypan blue assay?
03 March 2018 8,576 4 View
We have to dissolve GPNA (gamma-L-Glutamyl-p-nitroanilide; MW: 303.7) with proper solution. Datasheet notices this reagent is soluble in water (5 mg/ml), so we tried to dissolve this in water....
02 February 2018 3,243 2 View
We implanted specific tumor cells (10^7 cell)and after 3 days, I checked tumor (diameter : about 3 mm). However, from tumor injection to now, tumor size of all mouse was not changed . Is it...
12 December 2017 10,005 6 View
I puchased alpha tocopherol (cat.258024) in SIGMA-ALDRICH, but This product is oily solution. How to dissolve alpha tocopherol to use in cell culture?
08 August 2017 2,433 3 View
What is standard of resistant cell lines by specific drug ? I wonder if IC50 of resistant cell line is more 4 times than of original cell line, can the former be resistant cell line? (resistant...
05 May 2017 5,724 5 View
If drug inducing ROS or lipid ROS is treated dose-dependently in specific cells, level of ROS measurement by FACS increased dose-dependently?
05 May 2017 6,954 4 View
What is preferred method among MDA assay and FACS using BODIPY dye in measurement of lipid peroxidation? I want to know why, also.
05 May 2017 9,942 1 View
What are different positive controls in experiment measuring lipid ROS using BODIPY(581/591)?
05 May 2017 7,617 2 View
Does ROS or lipid ROS increase, if detached cell is treated by drug inducing ROS, despite of adherently growing cell originally ? Protocol of ab113851 DCFDA ROS kit recommends staining cell first...
05 May 2017 2,889 4 View
Why two mountains occur in dcfda assay through facs ? my protocol is following 1. detached cell using TE and drug treatment in HBSS media. 2. after appointed time (1,2 h), dcfda staining during 30...
05 May 2017 6,642 3 View
1 day, Cell seeding 2 day, Cell drug treatment After 24 h, we measured ROS to this method. 1) PBS wash, twice 2) 1/2 Trypsin/EDTA treatment, 5 min 3) After inactivating 1/2TE with FBS10% media,...
03 March 2017 7,532 4 View
30 ug protein Loading -> Transfer 300mA/1h PVDF 1. 5% BSA blocking 1hr 2. 1st antibody (pBeclin-1) 0.5ug/ml dilution in 2.5% BSA, O/N 3. TBS-T wash 5/5/5/10 min 4. 2nd antibody (rabbit) 0.2...
10 October 2016 8,260 4 View
If transfer time is long ( 300mA/ 2hr), proteins pass through PVDF membrane?
10 October 2016 720 3 View
