Which fluorescence wavelength should I choose?

06 July 2018 6,266 3 View

How to make DTT sample buffer in WB?

Hi, My name's Daiha Shin. I have 5x protein sample buffer (not including DTT). I don't know that 'how to make DTT-included sample in WB with upper sample buffer? I have tried to find this...

06 July 2018 7,968 0 View

Is it possible to dye two fluorescence at the same time ?

I'm preparing for immunofluorescence assay in live cell. Is it possible to dye two fluorescence at the same time ?

06 July 2018 8,714 2 View

How to treat GPNA drug in cancer cells?

Hi, everyone. I'm a master student, but I run into limitations about my study. How to treat GPNA drug in cancer cells? I treated GPNA drug in cancer cells via a leading journal, but almost all...

03 April 2018 4,732 2 View

Should you collect dead floating cells as well and harvesting live cells when performing the Trypan blue assay?

Should you collect dead floating cells as well and harvesting live cells when performing the Trypan blue assay?

02 March 2018 8,505 4 View

How to use drug working in high concentration ?

We have to dissolve GPNA (gamma-L-Glutamyl-p-nitroanilide; MW: 303.7) with proper solution. Datasheet notices this reagent is soluble in water (5 mg/ml), so we tried to dissolve this in water....

01 February 2018 3,172 2 View

How to experiment animal test of naturally regressed tumor or senescent xenograft in BALB/nu mouse?

We implanted specific tumor cells (10^7 cell)and after 3 days, I checked tumor (diameter : about 3 mm). However, from tumor injection to now, tumor size of all mouse was not changed . Is it...

11 December 2017 9,940 6 View

How to dissolve alpha tocopherol to use in cell culture?

I puchased alpha tocopherol (cat.258024) in SIGMA-ALDRICH, but This product is oily solution. How to dissolve alpha tocopherol to use in cell culture?

07 August 2017 2,348 3 View

Does ROS or lipid ROS increase, if detached cell is treated by drug inducing ROS despite of originally growing cell to be adherent ?

04 May 2017 2,799 4 View

Why two peaks occur in dcfda assay through facs ?

Why two mountains occur in dcfda assay through facs ? my protocol is following 1. detached cell using TE and drug treatment in HBSS media. 2. after appointed time (1,2 h), dcfda staining during 30...

04 May 2017 6,487 3 View

MD MOE RMSD

How to calculate the RMSD values for a MD simulation using MOE?

07 August 2021 0 0 View

MD MOE RMSD

How to calculate the RMSD values for a MD simulation using MOE?

07 August 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

What kind of fluid could I use for a pitfall trap that also does not invalidate molecular testing?

Hi, I want to start testing pitfall trap to obtain ants samples, but I need to conduct molecular analysis on those insects. So, what kind of fluid can I use? Ethanol expires too early and I need...

03 March 2021 5,978 5 View

Is there any test to determine the cardiovascular risk of patients with obesity? from the perspective of obesity?

We found tests to determine cardiovascular risk taking into account lipid profile, blood pressure, age, history of diabetes, other laboratories. But when weight is involved, there is a paradox. I...

02 March 2021 6,036 1 View

No title

02 March 2021 3,060 3 View

How to repeat an operation on an atom selection for multiple times in PyMol?

Hi, I am trying to construct a multi-layer fibril structure from a single layer in PyMol by translating the layer along the fibril axis. For now, I am able to use the Translate command in PyMol...

02 March 2021 4,569 4 View