What is preferred method among MDA assay and FACS using BODIPY dye in measurement of lipid peroxidation?
I want to know why, also.
Present study was showing this method in details in chapter tow
Thesis Correlation of Leptin and Resistin with Oxidative Stress in ...
I'm implementing RPA assay using fluorescence microscope. Protocol of its assay mentions that "The red fluorescence of RPA is excited at λ exc.=543 nm and collected through a long-pass filter...
06 July 2018 6,387 3 View
Hi, My name's Daiha Shin. I have 5x protein sample buffer (not including DTT). I don't know that 'how to make DTT-included sample in WB with upper sample buffer? I have tried to find this...
06 July 2018 8,100 0 View
I'm preparing for immunofluorescence assay in live cell. Is it possible to dye two fluorescence at the same time ?
06 July 2018 8,804 2 View
Hi, everyone. I'm a master student, but I run into limitations about my study. How to treat GPNA drug in cancer cells? I treated GPNA drug in cancer cells via a leading journal, but almost all...
03 April 2018 4,825 2 View
Should you collect dead floating cells as well and harvesting live cells when performing the Trypan blue assay?
02 March 2018 8,609 4 View
We have to dissolve GPNA (gamma-L-Glutamyl-p-nitroanilide; MW: 303.7) with proper solution. Datasheet notices this reagent is soluble in water (5 mg/ml), so we tried to dissolve this in water....
01 February 2018 3,262 2 View
We implanted specific tumor cells (10^7 cell)and after 3 days, I checked tumor (diameter : about 3 mm). However, from tumor injection to now, tumor size of all mouse was not changed . Is it...
11 December 2017 10,025 6 View
I puchased alpha tocopherol (cat.258024) in SIGMA-ALDRICH, but This product is oily solution. How to dissolve alpha tocopherol to use in cell culture?
07 August 2017 2,464 3 View
What are different positive controls in experiment measuring lipid ROS using BODIPY(581/591)?
04 May 2017 7,633 2 View
Why two mountains occur in dcfda assay through facs ? my protocol is following 1. detached cell using TE and drug treatment in HBSS media. 2. after appointed time (1,2 h), dcfda staining during 30...
04 May 2017 6,682 3 View
I have carried out MFC experiments on three different volumes, 50, 500 and 1000 mL of wastewater. Results after MFC treatment shows that TDS and EC are more in larger volumes of water i.e. TDS and...
09 August 2024 9,621 0 View
Hello everyone! I observed in my culture (htert-RPE1 cells) an orange- red particle at the bottom of the dish. It is visible to the naked eye as a very very small red dot. Could it be a...
09 August 2024 2,824 3 View
I am working in fungal fermentation of soybean meal and there is bacterial growth in them at times. I am trying to quantify fungal cell counts and bacterial cells; but I haven't been able to do at...
07 August 2024 7,535 4 View
Hi, I have a question about normalizing the MTT OD values for doing the statistical analysis. So, if we have 3 different plates and we call them 3 different replicates, so, first we would...
07 August 2024 8,106 4 View
Previously when I co-coluture anti-CD19(FMC63) CAR-Jurkat with Raji with E:T=5:1, Jurkat can eliminate Raji in 24h. However, when I test another CAR construct, although I can dectect totally CD69...
06 August 2024 641 2 View
To compare positive and negative cell populations in flow cytometry, should I compare unstained cells with antibody stained cells? Or with the isotype control? Most papers show comparison with...
06 August 2024 6,728 6 View
Hi, I know that low molecular weight (MW) molecules generally tend to have higher mobility, while high molecular weight molecules tend to have lower mobility. However, in my experimental...
06 August 2024 1,495 2 View
After immunohistochemistry of previously fixed in PFA and EtOH and then frozen 20 μm sections of zebrafish brain, DAPI staining is very weak (right) compared to the same sections stained without...
05 August 2024 9,637 2 View
Brain and body mass together are positively correlated with lifespan (Hofman 1993). The duration of neural development is one of the best predictors of brain size, and conception is the best...
05 August 2024 6,247 3 View
kindly reply me. Thanking you in advance.
05 August 2024 7,727 4 View