Is it possible to dye two fluorescence at the same time ?

More Daiha Shin's questions See All

Which fluorescence wavelength should I choose?

I'm implementing RPA assay using fluorescence microscope. Protocol of its assay mentions that "The red fluorescence of RPA is excited at λ exc.=543 nm and collected through a long-pass filter...

06 July 2018 6,387 3 View

How to make DTT sample buffer in WB?

Hi, My name's Daiha Shin. I have 5x protein sample buffer (not including DTT). I don't know that 'how to make DTT-included sample in WB with upper sample buffer? I have tried to find this...

06 July 2018 8,100 0 View

How to treat GPNA drug in cancer cells?

Hi, everyone. I'm a master student, but I run into limitations about my study. How to treat GPNA drug in cancer cells? I treated GPNA drug in cancer cells via a leading journal, but almost all...

03 April 2018 4,825 2 View

Should you collect dead floating cells as well and harvesting live cells when performing the Trypan blue assay?

02 March 2018 8,609 4 View

How to use drug working in high concentration ?

We have to dissolve GPNA (gamma-L-Glutamyl-p-nitroanilide; MW: 303.7) with proper solution. Datasheet notices this reagent is soluble in water (5 mg/ml), so we tried to dissolve this in water....

01 February 2018 3,262 2 View

How to experiment animal test of naturally regressed tumor or senescent xenograft in BALB/nu mouse?

We implanted specific tumor cells (10^7 cell)and after 3 days, I checked tumor (diameter : about 3 mm). However, from tumor injection to now, tumor size of all mouse was not changed . Is it...

11 December 2017 10,025 6 View

How to dissolve alpha tocopherol to use in cell culture?

I puchased alpha tocopherol (cat.258024) in SIGMA-ALDRICH, but This product is oily solution. How to dissolve alpha tocopherol to use in cell culture?

07 August 2017 2,464 3 View

What is preferred method among MDA assay and FACS using BODIPY dye in measurement of lipid peroxidation?

What is preferred method among MDA assay and FACS using BODIPY dye in measurement of lipid peroxidation? I want to know why, also.

04 May 2017 9,960 1 View

What are different positive controls in experiment measuring lipid ROS using BODIPY(581/591)?

04 May 2017 7,633 2 View

Why two peaks occur in dcfda assay through facs ?

Why two mountains occur in dcfda assay through facs ? my protocol is following 1. detached cell using TE and drug treatment in HBSS media. 2. after appointed time (1,2 h), dcfda staining during 30...

04 May 2017 6,682 3 View

Hello researchers Is this a random laser or just fluorescence?

I am using Rhodamine6G as gain medium and silver nanoparticles as scatterers on a microscope slide and laser input 532 nm comes from above.

09 August 2024 9,894 2 View

Weak DAPI staining after immunohistochemistry - how to improve?

After immunohistochemistry of previously fixed in PFA and EtOH and then frozen 20 μm sections of zebrafish brain, DAPI staining is very weak (right) compared to the same sections stained without...

05 August 2024 9,637 2 View

Could dyes amplify the spectrum of light to a specific wavelength?

I am interested to know the behavior of dyes toward light. Specifically, Blue dyes re-emit the spectrum, especially from the green zone (known as principal in LED lamps, and blue dyes are known...

05 August 2024 3,290 1 View

Are there any commercially available Donkey anti-Alpaca secondary antibodies?

Are there any fluorescently labeled anti-Alpaca secondary antibodies raised in Donkey? So far I have only been able to find anti-Alpaca secondaries raised in Goat. Or is this not possible due to...

04 August 2024 4,255 1 View

Why results of ROS flurescence are negative as there was no bacteria within?

Hello. I am working on ROS production of two systems: system A is cerium oxide and hydrogen peroxide, system B is cerium oxide nanoparticle, hydrogen peroxide and potassium bromide. I did some...

04 August 2024 5,974 3 View

How to calculate the molar proportions of the oxides from the XRF analyses?

02 August 2024 2,565 2 View

How to quantify the fluorescence intensity of only the merge area of an image using ImageJ software?

Dear All: I have to quantify the fluorescence intensity of cells that are fluorescent to two different markers, so i'm interested in finding out if this is possible. Previously I have...

22 July 2024 8,770 1 View

How to perform immunofluorescence on suspension cells?

Some time ago, I wanted to perform immunofluorescence on the S protein of mouse CD4 T cells using a Leica super-resolution microscope. I fixed with 4% paraformaldehyde, then added it to the well...

20 July 2024 3,797 2 View

How to determine LOD values?

I am performing fluorescence experiments using a ligand to detect metal ions. I want to determine the Lowest Detection Limit (LOD) using the formula LOD = 3σ / K. However, I'm uncertain about...

19 July 2024 1,086 1 View

Waht is the effect of isoelectric point on MB dye photodegradation by ZnO nanoparticles?

Recently I have submitted an article regarding MB dye degradation by ZnO nanoparticle. I received a suggestion to include the discussion on the effect of pH. I want to know what is the impact of...

19 July 2024 4,868 1 View