Hello! I am having a lot of trouble trying to calculate the concentration needed to make a 50 mL DMEM media + 50mM 2-Deoxy-D-glucose (2-DG) solution for my cell culturing experiment.
For reference, the media I am using is a DMEM 4.5 g/L glucose - https://ecatalog.corning.com/life-sciences/b2c/US/en/Media%2C-Sera%2C-and-Reagents/Classical-Media/Dulbecco%27s-Modification-of-Eagle%27s-Medium-%28DMEM%29/Corning%C2%AE-DMEM-%28Dulbecco%E2%80%99s-Modified-Eagle%E2%80%99s-Medium%29/p/10-013-CV
The molecular weight of 2-DG is 164.16 g/mol. The 2-DG that we have in the lab is a crystalline powder. I am trying to figure out which calculations I need to do to make this solution, but I am having a lot of trouble.
Hello Kristen Navarro
The molecular weight of 2-Deoxy-D-glucose is 164.16g/mol. So, one gram molecular weight (= 1 mole) is 164.16g. If you dissolve 164.16g of 2-Deoxy-D-glucose in a final volume of 1litre, you will have a 1M 2-Deoxy-D-glucose solution.
To get a 1M 2-Deoxy-D-glucose solution in a final volume of 50ml, you will have to weigh 8.2g of 2-Deoxy-D-glucose.
So, to get 50mM 2-Deoxy-D-glucose in a final volume of 50ml of DMEM media, you will have to weigh 0.41g (410mg) of 2-Deoxy-D-glucose powder and dissolve in 50ml DMEM media.
Best,
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