You should use differnt concentrations of a standard sample(oxaliplatin)and measure their coresponding absorption. Use the appropriate units and I suggest this number to be used as a concentrations
10, 3.3, 1, 0.33, 0.1, 0.033 and zero.
the atomic absorption photometer is measuring the free metal absorption of light at specific wavelength not in the liposomal form.
Then when you prepare the oxaliplatin in liposome form. Measure the sample with the photometer to know the amount of free metal in the sample using the calibration curve and compare it with the initial concentration that you start with. You can grt the effiency of entrapment
According to your needs, BOC Sciences can provide you with liposome encapsulation efficiency testing services based on high-performance liquid chromatography (HPLC) combined with molecular exclusion chromatography.