Problem in detection of a specific gene & elimination of primer dimer in qpcr?

In our lab, we are trying to do real time PCR for some genes. One of them has a size of 85 bp. Whatever we do, we had an amplification in negative control. When we run agarose gel, it always a...

14 February 2021 9,750 11 View

Can I use alpha amylase enzyme from (fungal source) if I need to characterize alpha amylase inhibitor from marine algae for hyperglycemia management?

I need to characterize alpha amylase enzyme from macroalgae for management of blood glucose level in diabetic patients, my question is can I use (fungal alpha amylase enzyme) in inhibition assay?

18 January 2021 735 4 View

How many times can we immunize mice before hybridoma preparation?

Dear all, Recently I have been working with monoclonal antibody production and I usually Immunize mice for a total of 4 times with the last one being diluted in PBS 3days before fusion...

09 January 2021 9,320 3 View

How to design manholes of a stormwater tunnel?

I am exploring for the design code or manual and standard drawings of access and maintenance manholes of the storm water conveying tunnels. (i.e free flow tunnels not pressurized ones) but all of...

22 December 2020 2,590 4 View

How to use TransDecoder for transcriptome analysis?

I am searching for new antimicrobial peptides and I don't have many skills in bioinformatics, so I want to know how to use these software packages to identify candidate coding regions within...

01 December 2020 4,632 3 View

Offshore steel structure of wind turbines 3D samples ?

i want to do a study on offshore wind turbines on sap model , initial study for graduation project of steel diploma (at ainshams university) , kindly if any specialized in this field can help me...

05 November 2020 3,571 4 View

SAS code for a "Split-plot in time" (based on CRD design)?

Dear all, I want to analyze a factorial split-plot in time using SAS. Factorial Experiment using Completely Randomized Design (CRD); Factor A: treatments (a1-a4) Factor B: harvest time,...

28 October 2020 3,515 4 View

Is there a solvent for blending PHB and Acid Ascorbic (via solution casting)?

Dear Researchers I want to add Ascorbic Acid (AA) to PHB in order to improve its biodegradability. AA is soluble in water but insoluble in those that are compatible with PHB (THF, Chloroform,...

07 October 2020 1,136 4 View

How can critical thinking be developed in scientific research?

Dear researchers, As I know, critical thinking is an essential feature that should be in scientist. Can you tell me how this skill can be developed? or how do you gain it from your long research...

16 September 2020 2,608 4 View

Journal vs conference publications, which one would you prefer ?

For sure journal publications have more weight than conference publications but i am asking about your preference. For example i like conferences a lot because i like to meet people and explore...

06 September 2020 7,468 42 View

It is possible to scan with an profilometer a round material with 10 mm thickness and 63,5 mm diameter?

The material is a composite with short unoriented fibers based on PMMA, ABS as a matrix, and fiberglass as reinforcement.

28 October 2020 2,890 2 View

What might be the reason for an apparent ever-increasing insulation resistance measured through successive DC voltage testing?

I have a very simple set up with two copper cylinders (30 mm diameter by 30 mm high) between which I place a thin layer (fiberglass 0.25 mm thick or mica 0.08 mm thick). Then I plug the leads of a...

06 March 2019 8,675 1 View

Any source for flexural design of above-ground composite pipelines?

I am currently doing research on pipelines subjected to bending loads. Do you know of any source to flexural design of above ground composite pipelines? Thanks

08 September 2018 3,356 5 View

What is a probable ratio of PU resin to GF Cloth in order to achieve high Young Modulus of a composite system?

I have been trying some ratio's of Polyurethane Resin to Glass Fiber Cloth but my young modulus results come up medium to low (15.000 MPa circumf. and 8-10.000 axial) and my Poisson ratio quite...

24 May 2018 9,014 2 View

Which options have been used for Passive-capillary wick samplers?

I am planning to test some wick-samplers in order to collect soil solution in some Andisols. In several researches it is mentioned that the material used as wick, corresponds to fiberglass, of one...

10 November 2017 9,303 1 View

Any database for the 9 moduli for FGRP and CFRP composites?

I need some database like we find on matweb.com for metals, but for composites. I need the 9 elastic constants especially for woven fiberglass and carbon fiber reinforced plastic, with any type of...

10 November 2017 1,975 5 View

Which is the safe temperature of fiberglass type range E? Not melting point

My application is in sleeves to protect electrical cables in high temperature water heaters.

04 May 2017 448 0 View

Does anyone have an experiences about FEA modelling of hygro-thermo-mechanical effects on the filament wound pipes ?

03 April 2017 3,511 2 View

Which Part of vehicle component that made of fiberglass which experienced fatigue failure?

31 December 2016 5,821 0 View

How much energy is needed for molding up 1 pound of fiberglass?

Could anyone please refer to a source (article, book, database, etc.) that shows/calculates energy for molding up 1 pound (or any other mass) of fiberglass?

05 June 2016 8,885 0 View