Mohammed A. A. Abdullah

14 Questions 10 Answers 0 Followers

Questions related from Mohammed A. A. Abdullah

Does the contaminated THP-1 cells secrete cytokines without stimulation?

I had an issue related to THP-1 cells which are contaminated with bacteria and when we test cytokines secretion they don't secrete any cytokine. Is that because of contamination of other factors...

07 September 2018 5,281 1 View

How I could make a scattered graph for single cell analysis like this?

I want to know which software that could make a scattered graph like that?

07 August 2018 1,544 2 View

What is the cell tracker dye with the highest fluorescence?

We culture cells on a microchip with a membrane that has autofluorescence and after few hours the stained cells start fading I am searching for a cell tracker dye with strong intensity to make it...

03 October 2017 312 3 View

What is the amount or concentration of Matrigel to culture cells on a microchip?

I want to culture cells on the microchip and I want to know how much of matrigel that I should use.

20 July 2017 3,728 1 View

What is the effect of the base on tetrazine?

I want to make coupling reaction between a compound with carboxylic group and the aminethat have tetrazine. I used HATU and DIPEA for the coupling for overnight. I get three spots may be the...

17 June 2017 9,568 2 View

What is the effect of temprature on the ribosomal cleavage actitiy?

What is the molecular property that makes the ribosomal activity increased by increasing temperature?

03 May 2017 8,967 1 View

What is the chemical structure of Dylight 488?

https://www.thermofisher.com/order/catalog/product/46402 I want to know the chemical structure of this dye Dylight 488

26 April 2017 4,429 2 View

How could I block the DNA non-spesific binding?

I am doing experiment using Antibody DNA conjugate but I noticed the DNA has many nonspecific bindings, How could I reduce them or block them? is there a specific sequence of DNA that doesn't have...

03 April 2017 8,648 1 View

What if I don't use copper in the reaction between linear alkyne and azide?

I need to have a ligation reaction with slow rate, I don't need the fast click reaction What is the rate of this reaction without copper is that work with some extent?

28 March 2017 319 1 View

How could we induce cell migration for cancer cell line of MDA-MB?

I am working on detection of some proteins secreted from cell associated with cell migrations and I try to notice migration of these cancer cells but they don't migrate. What I should add to these...

06 March 2017 3,808 4 View

What is the perfect parameters for urease enzyme for docking?

I use MOE to make docking for my newly synthesized compounds on urease enzymes but the enzyme is not totally parameterized How could I fix the problem?

01 April 2016 8,057 8 View

What is the difference between hydroxamic acid derivatives and carboxylic acid derivatives in metal chelation and chelation with metalloenzymes ?

I need references

24 February 2016 3,196 3 View

Where does NH2 appear in the NMR?

I make H NMR for hydrazide the NH appeared at 10.838 and I don't find the proton of NH2 group. I want to know where it is appeared, and I know it is exchangeable proton I know it may not be...

12 November 2015 3,856 2 View

How should I make a biological activity on Topoisomerase II enzyme with the 20 new compounds I have ?

any one could perform this biological Test??

07 June 2015 3,617 2 View