I am trying to understand how the expression of paralogues changed over time. I have real-time PCR data and gene tree. I want to reconstruct the estimated gene expression level at each node. I don't want this to be based on only a presence/absence score, like in morphological/categorical data, but want to have expression levels that are display continuous data. This kind of work has been done for transcriptomic surveys. Do you know whether/how it can be done for realtime PCR data?
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