In DFT or HF, is it more expensive to calculate the H matrix or to diagonalize it?

I was wondering: in common DFT (or Hartree-Fock) algorithms/codes, and in particular in solving the Roothan equation, what is the most computationally expensive part: 1- evaluate all the terms of...

01 February 2021 396 2 View

Suggestions for transfecting plasmids in ME-1 cells?

Hello everybody, I am currently working with recently purchased ME-1 cells. I was wondering if anyone that has experience transfecting this cell line, or even lines that are similar, would be...

08 October 2020 9,943 2 View

What is the mathematical relationship between degree of extraction and CO2/sample mass ratio in SFE?

In a supercritical fluid extraction system, what is the equation describing the relationship between the mass of extract or the degree of extraction (m extract/m extract(0)) and the amount of CO2...

07 October 2020 6,433 2 View

I require an experimental design that measures the percentage of successes in a trial rather than the average scores per trial?

Hi, What I am looking for is an experimental design for the scenario where a drug is given to randomized cohorts of patients and rather than measuring the average outcome for each case, I want...

15 September 2020 1,164 1 View

How to run a statistical comparison of same group and 2 conditions in fMRI?

Hi everyone, I have some doubts about which statistical test to apply in order to test the probability of eventA to happen at the same time of non-eventB. Basically I have my fMRI group with...

14 September 2020 1,887 3 View

Is there a difference between using potassium lactate or sodium lactate as preservatives in a product which will be used as intervaginal device?

If anybody could advise from medical or chemical prospector

23 March 2020 9,971 3 View

How can I effectively inoculate Pseudomonas syringae pv. actinidiae in young kiwi plants?

Hi everyone. I have to start a trial of efficacy of bacteriophages against the kiwi bacterial canker, but before I need an effective method to inoculate my 15 6 months old kiwi plants with...

24 November 2019 383 3 View

Is there a device which can dillute a gas-sample before it enters a continious-flow gas-analysator?

I want to measure gaseous NH3 emitted from slurry with the gas-analysator G2508 from Picarro. I expect the concentration to be ten and more times higher than the operating range of the...

02 October 2019 8,276 3 View

How to test single hypothesis with various observation samples?

Hello, I'm conducting OLS regression in order to find value drivers for abnormal returns. My model includes 6 different independent variables, and i'm forming 20 subsamples (observations). My...

03 April 2019 272 1 View

What statistical test can I use to test hypotheses with a limited sample size?

For my bachelor thesis, I'm seeking to prove that the degree of adoption of value based pricing strategy has a positive effect on the innovation performance of SMEs. At first, I thought to use a...

05 June 2018 8,364 17 View

Why does blocking with my immunogenic peptide cause nuclear fluorescence in ICC?

Hi all, I am validating a polyclonal antibody raised against human ANT4, through WB and ICC. In most of my controls, the antibody shows very little off-target binding, but when I pre-incubate the...

03 March 2021 5,999 3 View

Does Napabucasin soluble in dichloromethane and in ethanol?

I need to prepare a solution of Napabucasin (inhibitor of cancer cell stemness) in dichloromethane. The drug is expensive, so maybe somebody already tried to do it. If it is soluble, what is the...

02 March 2021 9,945 3 View

Help request in CST microwave?

im working now on TPV solar cells and metamaterials and i realy need our help in CST microwave i have a tpv cell simulation and i want to extract particullarly the absorbtion of only the active...

02 March 2021 2,246 2 View

Dapi fluorescence protocol for colon cell lines ?

28 February 2021 2,534 2 View

Does RNAiMAX transfection reagent work in serum free medium?

I diluted siRNA and RNAiMAX in opti-MEM and added to the cells which they were in the growth medium. Is it a right way? or should I culture cells in the opti-MEM medium for a while and not in...

26 February 2021 10,041 3 View

Has anyone had luck using cell lines other than HEK 293 (such as COS1) to package lentivirus?

I am looking to package lentivirus in endothelial cells but I am opposed to using 293 cells due to personal ethics regarding the source. I have done some reading and saw that COS1, COS7, & CHO...

25 February 2021 3,030 1 View

Unknown debris sheets observed in cell culture flasks?

Hi, We culture neural progenitor cells in T175 culture flasks coated with PLO-Laminin. We have successfully grown these cells with our protocol for months. Recently, we observed large sheets...

25 February 2021 3,443 4 View

How do I transfect a large plasmid into PC12 cells with nerve growth factor (NGF) receptor?

I have a large 16 kb plasmid, which I need to transfect into PC12 cells. Lipofectamin 2000 didn't work and with GFP alone the transfection rate is very low. I also tried the neon invitrogen...

25 February 2021 6,635 5 View

What is a good reagent for lentiviral plasmid transfection in HeLa cells?

I have been using Lipofectamine 2000 but was hoping there may be a better protocol or one for something like Lipofectamine PLUS reagent.

24 February 2021 1,633 4 View

Is it possible that Cancer cells could survive in heparinized blood for 48 hours?

Hi everyone, I am just wondering if we incubated 200 microliter of whole blood with 50 microliter of cancer cells(1bout 5000cells) do you think that cancer would survive for 48 hours?

23 February 2021 3,840 3 View