Tasshitra Subramaniam

4 Questions 6 Answers 0 Followers

Questions related from Tasshitra Subramaniam

Why I'm unable to observe insert sequence bands in Colony PCR?

Hi, I have grown BL21 cells with the pET28a that carries my insert sequence. It is growing on the selection plate but I can't observe any insert sequence bands when I do colony PCR. Is there any...

16 July 2024 5,125 5 View

How to induce substitution mutation to a 3D Protein in silico?

Hi, I am working on protein-protein interaction studies, specifically on antibody-antigen interaction. I would like to observe the changes in interaction if there's mutation occurs in the protein....

05 October 2023 8,782 3 View

How to avoid ligand and receptor chain from merging into one chain after molecular docking?

Hi all, I've performed molecular docking using ClusPro. One of the chain from the ligand merged to a chain from the receptor when I wanted to study the protein-protein interaction using iCn3D....

03 July 2023 3,700 4 View

How to identify the binding sites of the antigen using GROMACS?

Hi, I am working on protein-protein interaction studies, specifically on antibody-antigen interaction.

20 June 2023 9,844 5 View