concerning the induction of dendritic cells (DCs) apoptosis by tumor supernatant cancer. I know from many papers that tumor supernatant (from different types) has ability to induce apoptosis to dendritic cells , but when I use MCF-7 supernatant (collected after complete sheath) to induce apoptosis using annexin test ,,, I get nothing ...MCF-7 supernatant did not induce apoptosis in DCs...this contrary to previous studies.. why this with me ? What is the wrong? Could you help me please?