I am culturing Human lung fibroblasts (HFL) in DMEM supplemented with 10% heat-inactivated FBS, 1% MEM non-essential amino acids, 1% penicillin-streptomycin, and 1% L-glutamine. However, they have very slow growth (around 10-15 days to reach confluence in T25).
Is this normal? What can I do to improve their growth rate?
Thanks!
Malcolm Nobre
Hello Berina
You are right. It takes roughly around 10-12 days for human lung fibroblasts to reach confluency. Once you see smaller colonies forming in the flask just passage them in to a Petri dish of smaller area. What is the solution you use for trypsinization ?
Thank you.
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