Hello!

I am trying to isolate exosomes using 2 approaches: PEG and size-exclusion chromatography. Afterwards I would like to perform microscopy analysis in order to confirm presence of exosomes in my isolates.

Which protocol do you suggest me for sample preparation for SEM and/or AFM microscopy?

PS. My samples were eluted in SEC by PBS. Am I going to have some interference caused by PBS in this case?