Berina Šabanović

3 Questions 2 Answers 0 Followers

Questions related from Berina Šabanović

How to increase the growth rate of Human Lung Fibroblasts?

I am culturing Human lung fibroblasts (HFL) in DMEM supplemented with 10% heat-inactivated FBS, 1% MEM non-essential amino acids, 1% penicillin-streptomycin, and 1% L-glutamine. However, they...

18 November 2020 5,896 3 View

Size-exclusion chromatography of exosomes after PEG precipitation?

I am trying different methods of isolation of exosomes to validate the most convenient one for further studies. I have used the protocol for precipitation with PEG (30%) and now I am considering...

12 December 2018 9,835 4 View

Sample preparation for SEM and/or AFM of exosomes?

Hello! I am trying to isolate exosomes using 2 approaches: PEG and size-exclusion chromatography. Afterwards I would like to perform microscopy analysis in order to confirm presence of exosomes...

26 November 2018 3,473 1 View