I am trying different methods of isolation of exosomes to validate the most convenient one for further studies.

I have used the protocol for precipitation with PEG (30%) and now I am considering to do size-exclusion chromatography with Sephadex CL-4B.

My question is following: Is it necessary to remove PEG (if yes, is it better to perform some washing step or ultracentrifuge) or I can load the sample as it in columns?

Thank You!

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