David E. Bautista-Erazo

12 Questions 93 Answers 0 Followers

Questions related from David E. Bautista-Erazo

Problem with ion suppresion in HPLC-MS (curcumin, mouse plasma)?

We want to determine curcumin in mouse plasma after subcutaneous administration of some formulations. The idea was to perform the determination by HPLC-MS. However, we have been having a problem...

11 July 2023 4,068 2 View

Way to visualize curcumin in mice tissues with some specific stain?

Is there a way I can visualize curcumin in mice tissues (for example after subcutaneous administration of a curcumin formulation) employing some sort of specific stain for curcumin or polyphenol...

24 May 2023 9,758 2 View

Has anyone used the Hooke Labs kit to induce EAE? How recommendable is it?

In our laboratory, we have standardized the EAE model with MOG. However, the last time we did an experiment, there was a problem and the control mice did not get sick. We did the troubleshooting...

26 January 2021 7,670 0 View

Which is the best kit for removal of endotoxins from a recombinant protein?

Hi. In our laboratory, we produce a recombinant protein in E. coli, but it is generated very contaminated with endotoxins. We want to remove these endotoxins. We have used a Pierce kit called...

25 April 2019 9,205 13 View

Which solvents are useful to dissolve liposomes (lipid vesicles) with protein without precipitating the protein?

Hi. I'm encapsulating a protein inside a liposome (lipid vesicle). I want to quantify encapsulated protein by UV since it absorbs and the lipids don't. But I need to dissolve both the liposomes...

05 April 2019 3,810 10 View

What would be the best approach to perform a determination of curcumin in mice organs (biodistribution assay) by fluorescence spectroscopy?

Hello. In my laboratory we need to perform the quantification of curcumin in some mice organs after subcutaneous administration of that molecule (“biodistribution” bioanalysis). Our experimental...

04 August 2018 994 2 View

Which are the best methodologies to extract protein from liposomes?

We are trying to quantify protein encapsulated in liposomes. Since lipids in BCA interfere with the measurement, we need to perform a extraction and get rid off the lipids but with a good recovery...

01 August 2017 2,282 5 View

Getting rid of particulate impurities in chloroform?

I have 500 mL of chloroform in a Schott flask but it's getting kind of full of some particulate impurities. I want to filter it. What's the best procedure to mantain the integrity of the organic...

02 March 2016 5,473 4 View

Can solvents used in histopathology dissolve substances that I need to observe in a specific mice tissue?

We need to observe a compound (curcumin) in the footpad of a mouse. Solvents used for histopathology are formaldehyde and nitric acid. In addition, we have to decalcify the bone in order to do the...

02 February 2016 8,664 4 View

How can I remove phospholipids from HPLC C18 columns?

I am analyzing liposomes in HPLC. I want to remove phospholipids from my C18 column in order to extend its lifetime. What solvents do you suggest? Thanks.

26 August 2015 3,969 8 View

How can I separate vitamin D from phospholipids in order to analyze vitamin D by HPLC-UV?

Hello. I am making Vitamin D-liposomes. In order to calculate loading efficiency I have to measure vitamin D in the bilayer of the liposomes. I need to separate phospholipids because they damage...

25 August 2015 4,839 4 View

How to employ DLS, microscopy and UV spectrophotometry to evaluate disruption of liposomes with detergents?

We are preparing liposomes encapsulating a protein. We want to calculate the encapsulation efficiency of the protein inside the liposomes. We employ centrifugation to separate large liposomes (the...

01 January 1970 4,209 15 View