23 Questions 194 Answers 0 Followers
Questions related from David E. Bautista-Erazo
We want to determine curcumin in mouse plasma after subcutaneous administration of some formulations. The idea was to perform the determination by HPLC-MS. However, we have been having a problem...
11 July 2023 4,146 2 View
Is there a way I can visualize curcumin in mice tissues (for example after subcutaneous administration of a curcumin formulation) employing some sort of specific stain for curcumin or polyphenol...
24 May 2023 9,853 2 View
In our laboratory, we have standardized the EAE model with MOG. However, the last time we did an experiment, there was a problem and the control mice did not get sick. We did the troubleshooting...
26 January 2021 7,820 0 View
Hi. In our laboratory, we produce a recombinant protein in E. coli, but it is generated very contaminated with endotoxins. We want to remove these endotoxins. We have used a Pierce kit called...
25 April 2019 9,295 13 View
Hi. I'm encapsulating a protein inside a liposome (lipid vesicle). I want to quantify encapsulated protein by UV since it absorbs and the lipids don't. But I need to dissolve both the liposomes...
05 April 2019 3,917 10 View
Hello. In my laboratory we need to perform the quantification of curcumin in some mice organs after subcutaneous administration of that molecule (“biodistribution” bioanalysis). Our experimental...
04 August 2018 1,112 2 View
Hello. I want to standardize a platform to prepare micrometric (900-1200 nm) drug nanosuspensions (nanocrystal dispersions), at my laboratory, of curcumin and other hydrophobic drugs. I have read...
15 June 2018 3,469 2 View
I've been searching for cationic materials to stabilize a suspension of drug nanocrystals. We want to prepare curcumin nanocrystals (with a bottom up approach, probably) but we need them to be...
19 April 2018 1,572 5 View
We are trying to quantify protein encapsulated in liposomes. Since lipids in BCA interfere with the measurement, we need to perform a extraction and get rid off the lipids but with a good recovery...
01 August 2017 2,343 5 View
We are preparing liposomes (900 nm) of a recombinant protein (55 kDa). After the extrusion of the liposomes, we performed 2 centrifugations to separate our larger liposomes from small liposomes...
29 June 2017 8,351 17 View
We want to analyse liposomes in a SEM. But, since liposomes are unstable during drying procedures, our liposomes look ugly when subjected to high vacuum. We want to explore any fixation method...
03 February 2017 4,662 6 View
We are developing dexamethasone sodium phosphate encapsulated in cationic liposomes. We are having some stability problems. It seems that our zeta potential is +20mV which is not okay for us....
26 January 2017 9,984 5 View
We are trying to elucidate if we need fixation in order to perform TEM of a sample of liposomes. Do you think it's fundamental? Or can we skip that step and perform the uranyl staining of the...
18 October 2016 8,911 9 View
If I have the same liposomes of two different sizes, i.e. 200 nm vs 1000 nm, but the composition is the same, both of them should have the same zeta potential? Thanks.
22 June 2016 2,963 5 View
We are producing curcumin liposomes and when we are forming the thin lipid film, the solution of curcumin+DSPC+stearylamine in chloroform changes its color from dark yellow to black when the...
03 May 2016 8,763 18 View
I have 500 mL of chloroform in a Schott flask but it's getting kind of full of some particulate impurities. I want to filter it. What's the best procedure to mantain the integrity of the organic...
02 March 2016 5,543 4 View
I'm trying to separate the encapsulated active compound from the non-entrapped active compound in my liposomes. Do you have a protocol or some recommendations about dialysis? Centrifugation failed...
01 March 2016 5,607 7 View
I am preparing liposomes using the film hydration method (Bangham). However, I need liposomes over 400 nm (size) with a good polydispersity. In my protocol, after hydration of the lipids with PBS,...
03 February 2016 2,512 10 View
We need to observe a compound (curcumin) in the footpad of a mouse. Solvents used for histopathology are formaldehyde and nitric acid. In addition, we have to decalcify the bone in order to do the...
02 February 2016 8,792 4 View
I am using chloroform in my preparation of liposomes. Is it a big a problem during the scaling up? Thanks.
26 January 2016 6,847 19 View
I am analyzing liposomes in HPLC. I want to remove phospholipids from my C18 column in order to extend its lifetime. What solvents do you suggest? Thanks.
26 August 2015 4,039 8 View
Hello. I am making Vitamin D-liposomes. In order to calculate loading efficiency I have to measure vitamin D in the bilayer of the liposomes. I need to separate phospholipids because they damage...
25 August 2015 4,919 4 View
We are preparing liposomes encapsulating a protein. We want to calculate the encapsulation efficiency of the protein inside the liposomes. We employ centrifugation to separate large liposomes (the...
01 January 1970 4,282 15 View
