Dr. Pankaj K. Bagul

4 Questions 37 Answers 0 Followers

Questions related from Dr. Pankaj K. Bagul

Where do I get oligonucleotide probes for EMSA assay? How to design the probes for transcription factor of interest?

I am planning to do EMSA assay with my samples for some known and some new transcription factor. How do I get the oligonucleotide probes for the assay? Somebody please tell me where I will get...

07 July 2013 4,615 1 View

Use of Coomassie blue stain and ponceau stain as loading control in western blot?

Is it reliable to use coomassie staining (before transfer) or ponceau staining (after transfer) as a representation of loading control? As most people get problems in selecting a good loading...

04 April 2013 3,863 28 View

How to identify transcription factors binding to a specific DNA sequence?

I have an idea to identify the transcription factors binding to a specific DNA region. I don't have any transcription factor candidate, only what I have is a potential promoter region of a gene....

04 April 2013 759 32 View

Can anybody please tell me the rules or ethics behind publishing western blot results?

It seems to be very difficult to follow a proper pattern of publishing the western blot data. Everybody follows different patterns (ex. in the no. of samples or etc.). Somebody please tell me...

02 February 2013 2,082 5 View