Dear all,
could anyone suggest suitable markers to detect necroptotic cell using fluorescent microscopy?
I saw that proteins typically used for this are RIPK1, RIPK3 and MLKL. But for each there is a phosphorylated version, so I'm not sure which to buy.
Thanks!
Dear @Marcel
This topic addresses different methods for assessing and moitoring necroptotic cell under fluorescence microscopy
Dear Marcel!
Please You look at these article:
Article Biomarkers and Molecular Probes for Cell Death Imaging and T...
Table 1
Cell Death Biomarkers and Probe Targeting Groups
Necrosis Biomarkers
Much attention has been focused on developing biomarkers for necroptosis imaging since it has an important role in numerous biological and pathophysiological settings (Article Molecular mechanisms of necroptosis: An ordered cellular explosion
attached)
MLKL is phosphorylated by RIP3/RIPK3.
Phospho-MLKL at Ser358 and Thr357 is the most precise protein to confirm necroptosis via RIPK3-mediated activation of MLKL.
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