Hi there,

I am looking for a protocol to isolate RNA using low total CFU of bacteria (S. Aureus). We already have a good isolation protocol for mammalian cells (cell culture and tissue), but now we also want to isolate RNA from bacteria. We already found several protocols, but these protocols are all based on high CFU numbers. Like a 50 ml culture of a 1x10^8 CFU/ml. For our experiments, we want to isolate RNA from only 1 ml culture with a concentration ranging from 1x10^6 - 1x10^8 CFU/ml. We have tested several things, but our yields are just too low to use for downstream applications. Hopefully, you one you has an answer for us that works.

Thanks in advance..