How to construct networking pathway using own miRNA with pre selected genes?

Dear experts, I have a list of miRNA and corresponding target genes for which i want to construct pathways and network interaction, so can you please suggest me any tool or software which will...

31 December 2017 7,736 4 View

Is there any insilico tool available for pathway analysis??

Dear members, i have a list of genes which intersects in 20 different pathways, so i wish to generate a picture view or flow diagram view which explain number of pathways originated from a single...

01 February 2016 8,009 5 View

Does anybody have ePath 3D software?

Dear members, I begins to draw molecular diagrams which includes organelles and pathways, i used cell designer, cell illustrator and vector draw softwares, but recently i come to know ePath 3D...

07 August 2015 7,100 2 View

Which cell lines in prostate,breast and pancreatic cancer contain wild type P53?

06 July 2015 4,094 3 View

How to overcome from a fungus contamination in a cell culture?

If any one is facing fungus contamination or other due to eviormental conditions then what precautions are necessary and how he can prevent contamination?

31 December 2014 7,242 10 View

Is there a database available which contains data on miRNA-targeted genes in specific diseases with the validated gene on that particular organ?

Let's suppose hsa-miR-320 was found to be deferentially expressed in ESRD (end stage renal disease) - how can we predict its exact gene in the renal cortex?

10 November 2014 7,018 13 View

MiRNA qpcr problem?

Dear All, mirna primer showing some problem in the melting curve? any idea why? As attached is the melting curve. The forward sequence is obtained from miRBase and reverse primer is universal.

28 February 2021 5,008 4 View

Can MicroRNA cross the blood brain barrier?

In diseased conditions, many genes and gene products are visible in the bloodstream. So my question is whether we can find traces of microRNA which is involved in disease and disorder in blood serum?

24 February 2021 7,064 3 View

Are exogenous molecules transferred between human cells during doubling ?

Hello RG community, I wonderer whether exogenous molecules (e.g. reporter plasmids, miRNAs or other artificial molecules) are transferred between cultured human cells during their doubling ? So,...

21 February 2021 6,633 3 View

Has anyone encountered any problems with abm good microRNA primers for quantification of mature miRNAs?

I've been trying to quantitate mature microRNAs in 293T cells with and without 'X'. 2ug of total RNA (isolated by Trizol method) is converted to cDNA using All-in one microRNA cDNA synthesis kit...

17 February 2021 8,986 2 View

Does anybody know the ways of homogeneous ripening of Mung bean crop?

Some crops (Mung bean) are matured at different times i.e., harvested at several times that's why the labour cost of the farmer is high. So, could you please, is there any ways or chemicals are...

12 February 2021 294 4 View

How much could knowing the maturity level of an organization contribute to a given process?

The doubt is raised because there are several methodologies to estimate the degree of maturity of certain processes, such as computerization, communication, human resources, among others, and...

03 February 2021 4,753 7 View

What is the best way to fix plasma derived miRNA for subsequent cDNA synthesis?

I need to quantify blood plasma derived miRNA through q-RT-PCR. For this I need to fix RNA content in my cDNA reaction. What is the best way to do this? Currently, I take readings of my plasma...

03 February 2021 9,330 3 View

What is the general miRNA yield one gets from the Qiagen miRNEASY serum/plasma advanced kit?

I am using the Qiagen miRNEASY serum/plasma advanced kit, using 400ul of frozen plasma. What is the yield you get on average? I am using nanodrop currently for quantification. I am getting...

02 February 2021 8,806 2 View

Which miRNA arm is a right target for analysis, 3p or 5p?

I'm working on selecting mouse miRNAs to analyze and stumbling upon one confusing issue, some of the papers do not specify which arm they analyzed and they just state something like " miR-34a was...

16 January 2021 3,792 4 View

Is negative controls necessary for western blotting?

I am trying to confirm my miRNA target by western blotting. I have a ds mimic and a ss inhibitor of my miRNA. Is it necessary to use negative controls for mimic and inhibitor? If yes, how can I...

09 January 2021 2,320 3 View