How to construct networking pathway using own miRNA with pre selected genes?

Dear experts, I have a list of miRNA and corresponding target genes for which i want to construct pathways and network interaction, so can you please suggest me any tool or software which will...

31 December 2017 7,736 4 View

How to identify microRNA binding location in particular gene?

Dear Experts, I have a list of some human mature miRNA, for which i had corresponding target genes, but i am unable to find out that in which location miRNA bind to corresponding gene, whether in...

31 December 2017 5,833 4 View

Is there any insilico tool available for pathway analysis??

Dear members, i have a list of genes which intersects in 20 different pathways, so i wish to generate a picture view or flow diagram view which explain number of pathways originated from a single...

01 February 2016 8,009 5 View

Which cell lines in prostate,breast and pancreatic cancer contain wild type P53?

06 July 2015 4,094 3 View

How to overcome from a fungus contamination in a cell culture?

If any one is facing fungus contamination or other due to eviormental conditions then what precautions are necessary and how he can prevent contamination?

31 December 2014 7,242 10 View

Is there a database available which contains data on miRNA-targeted genes in specific diseases with the validated gene on that particular organ?

Let's suppose hsa-miR-320 was found to be deferentially expressed in ESRD (end stage renal disease) - how can we predict its exact gene in the renal cortex?

10 November 2014 7,018 13 View

Can linear DNA be used as template for site-directed mutagenesis?

Is it possible to induce site-directed substitution mutation by quick-change method on linear dsDNA? or it has to be cloned in some vector? If yes, should it be treated with the Dpn1 enzyme...

03 March 2021 401 4 View

Does any one know how i can prepare liver tissue with transmission electron microscope with good fixation?

i have problem in preparation of liver tissue with bad cell organelles and how i can adjust osmolarity

03 March 2021 8,890 1 View

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02 March 2021 3,060 3 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

Do you need the CMV enhancer before the CMV promotor for a proper function of CMV on the gene that follows?

I'm a student and I have to produce a cell line with a knockin for the NRF2 gene with GFP. I have to put a promotor in front of the GFP because the gene will be too far away from the promotor of...

28 February 2021 7,127 2 View

What could be some reasons why unstimulated monocytes are getting activated?

Im doing PBMC isolation -> CD14+ enrichment using magnetic beads -> stimulation setup. My negative control is just cells in cRPMI but they seem to get activated over and over again.

28 February 2021 7,883 3 View

PUC19 as a transfer plasmid for lentiviral delivery?

Hello, Is it possible to use pUC19 as a transfer vector to be packed in using the second generation viral particles packaging system( pMD2.G; psPAX2 plasmids)? As far as I understand it there is...

28 February 2021 4,868 2 View