Look for cell adhesion, expression of CD68 and ability to phagocytize beads or killed bacteria.

Thank you for the answer Jordan, but what about the expression of CD163? it that any use of?

You can detect the Macrophages adhesion under microscope. They will stick to the well plate or tube.

To release this adhesion you can use trypsin.

Very Best

I agree with Jordan: monocyte morphology change dramatically after macrophage differentiation, the former looks like a round cell and the latter tend to spred with cytoplasmic looking pseudopods. A function test without doubt is phagocytocis of latex beads, red bllod cells, or CANDIDA and Staphiloccoci bacterial cells. You may also use flow cytometry using fluorochrome label monoclonal antibodies against macrophage CD markers. Good luck.