I would like to know about the best offline tool for guide RNA designing?

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Does the China's adaption to GM soybean Technology contradicts to its practice of virtual water trade policy?

Recently, the Ministry of Agricultural and Rural Affairs enacted the approval of twelve genetically modified (GM) soybean varieties, marking a strategic pivot in China's quest for food security....

05 June 2024 1,143 0 View

Protein hits low with Hela QC for QExactive HFX?

Hi, I have a QE HFX and last winter the protein hits dropped and the field engineer found all evaluations on the MS passing but hits did not improve until the electronics board was replaced. RF...

26 March 2023 1,256 0 View

What are the geriatric research project funding agencies receiving application for funding on regular basis?

funding agencies for geriatric research projects

16 August 2022 1,380 3 View

Hypotheses and Recent field: Is it possible transmission of Leptospira Spp or Borrelia Spp to poultry from exposure to the big rat bandicoot ?

I wand to know. Recently recorded higher mortality in broiler birds due to consume that big rat bandicoot contaminated water.

07 July 2018 8,722 2 View

THP I cell line is not growing, any suggestions?

My THP I cell line doesn't seem to show the full growth pattern now. It is not reaching confluency even after 72 hrs of incubation. It showed a full potential growth in the initial stage. But...

22 January 2018 3,161 1 View

How to identify macrophages after transformation from monocyte in cell culture?

Any method including morphological analysis.

13 November 2017 4,674 4 View

Is there any relationship b/w weight of the structure and seismic resistance(lateral displacement resistance)

As i am design and analysing the industrial structure made up of steel and cold form steel. if i am using cold form steel the wt of the structure gets reduced but the lateral displacement value...

11 April 2017 8,919 4 View

I analyse my industrial structure with K bracing under a seismic effect in staad pro. it shows lots of instability how do i solve it?

i am comparing the results of various bracing system for industrial structure. while i analysing the structure with X and diagonal bracing it shows less lateral deflection 7-18 mm and 4.73-8.95...

22 March 2017 8,929 3 View

What is the best technique to extract lipids from yeast cells?

I have tried different procedures for yeast cell lysing and extracting lipid from it, but the yield was observed to be very meagre. Can anyone suggest me the best method for yeast lysis and...

22 June 2016 2,841 1 View

Why there is drop in OD in culture of bacillus subtilis observed in fermenter?

OD REACHES 27-30 then it suddenly crash down to 7od. It never behaved like this. I just need to know if anyone experienced the same or does anyone know the answer.

18 May 2016 6,408 2 View

Is there a problem with my RNA pellet?

Hello, I am currently having problems with RNA extraction. I am using mouse liver (C57BL6J), and I have extracted RNA from mouse liver before. Before this experiment, my final RNA pellets were...

11 August 2024 7,082 3 View

Strugglling with m6A dot blot any suugesstion ?

I have been doing the m6A dot blot for a while with no improvement, I am extracting the RNA, and I can see the dots although the three biological replicas give a different reading on the memberan...

10 August 2024 8,539 5 View

RNA Extraction Using Hot Borate Method No Longer Working?

I've been performing RNA extraction on cotton petiole tissue for a few months now using the method described in the following paper, a derivative of the typical hot borate method...

08 August 2024 9,882 2 View

Does Anyone have expertise in in vitro transcription and RNA pull down assay?

I am currently working on LncRNA; to know the lncRNA-protein interactions I want to do RNA pull down assay, so I need to design primers with T7 promoter. I need assistance in this regard.

07 August 2024 6,622 1 View

E.coli contamination in human RNA seq data ?

Recently, we observed that 99% of the sequences in our RNA-seq data corresponded to the E. coli genome. Despite multiple DNAse treatments after RNA extraction and ribosomal depletion, we were...

06 August 2024 807 3 View

RNA later for the preservation of RNA in fecal samples at room temperature for one day (37°C)?

I am planning to collect human fecal samples for metatranscriptomic analysis using MGI. These samples are from indigenous people living in a region with high temperatures. I will have access to a...

06 August 2024 1,367 3 View

Do you have good tips for seaweed tissue preservation in the field for post RNA extraction?

I will be with my students collecting seaweed samples in a marine farm and later we will process this tissue for RNA isolation and further sequencing. Does anyone have tips on how to collect the...

04 August 2024 501 2 View

What is the acceptable p-value cutoff for GO enrichment analysis ?

I have an RNA-seq data that I have analysed using Limma-voom and have extracted the gene IDs, log2FC and the p-values. At p value < 0.05, I have over 10,000 DEGs, however, when I run the GO...

31 July 2024 225 2 View

Inquiry on Maximum Nucleic Acid Volume for 2.5 mL Liposome Solution?

I am currently working on a project involving liposomes and need to determine the maximum volume of siRNA that can be added to a 2.5 mL liposome solution with a total lipid concentration of 10...

30 July 2024 6,420 1 View

How to prediction 2D to 3D aptamer?

Hello, I have a little problem. Well, right now I only have the DNA aptamer sequence. I went to see the 2D model form with DNA folding. After that, I used RNA Composer to simulate in 3D and used...

30 July 2024 6,794 0 View