How to identify a virus through a spacer(CRISPR)?

16 December 2021 1 6K Report

Say I have a bacterial genome, and I have located the CRISPR and spacer regions in the genome. Is there any way to find out the phage(s) that infected this bacteria by using those spacers?

Would you please recommend databases and tools to do the same?

Shivam Maurya

CRISPR “spacer” sequences are transcribed into short RNA sequences (“CRISPR RNAs” or “crRNAs”) capable of guiding the system to matching sequences of DNA. When the target DNA is found, Cas9 – one of the enzymes produced by the CRISPR system – binds to the DNA and cuts it, shutting the targeted gene off Anurup Mohanty

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