How to increase the final DNA yield using the Qiagen PowerSoil DNA extraction kit?

Hi, I am having troubles trying to extract DNA using the Qiagen PowerSoil kit from cave sediment (mud/clay). I always get very low yield:

03 January 2021 5,049 1 View

Can all iron oxide nanoparticles be considered superparamagnetic?

Dear researchers, Can all iron oxide nanoparticles be considered superparamagnetic? Or the "superparamagnetic" definition can be assigned only to certain types of iron oxide nanoparticles? If so,...

26 October 2020 546 5 View

Do you know how long it takes for a polyprepylene surgical mask to degrade?

I'm a master student in the university of Geneva. I have to find a scientific paper of the degradation of the surgical mask to add it in my master's thesis. I only found non-scientific journals...

03 September 2020 2,940 3 View

Piecewise multiplicative scatter correction in R?

Anyone is aware of an R package available online to perform a Piecewise Multiplicative Scatter Correction (PMSC)? This technique is an advance of the Multiplicative Scatter Correction (MSC)...

23 August 2020 2,893 2 View

What is the best chromatography (type, resin..) to separate blood lipoproteins among each other?

Dear all, I am trying to understand which is the "golden standard" method to separate lipoproteins from the blood (LDL, HDL, IDL, chylomicrons...). I found some methods online, and apparently...

10 August 2020 4,738 5 View

Latent Class/Profile Analysis AND prediction of binary outcome variable?

Hey everyone, I am trying to conduct a mixture of a latent class/ profile analysis because I have categorical and continuous outcome variables. (1) how can I combine these two? I would also...

26 March 2020 354 2 View

DdRAD final library shows low concentration at size selected peak. Anyone have any idea what could have gone wrong?

I sent off my first ddRAD library for sequencing and as QC it was first run through the bioanalyzer. I size selected with a pippin prep between 375-475 bp and the results of the bionalazyer show a...

12 January 2020 9,403 1 View

Special issue on social farming. Would do you like to contribute?

Dear all, I am Valentina Cattivelli and I am a guest editor at Sustainability (IF 2.5). With this, I would like to invite you to submit a paper within a special issue "Social farming for Social...

10 November 2019 5,392 13 View

Where to find good description of a cognitive and language development test standardization?

Hello everyone, I am new in the area of Developmental Psychology and I am looking for a good description of the standardization process of a test for cognitive and/or language development. For...

28 October 2019 2,346 4 View

How to separate human from bacterial RNA?

I extracted RNA from human saliva samples but the amount of bacterial (basically not human) RNA is very high: despite the high RNA concentration (Nanodrop quantification), after real-time PCR...

22 July 2018 4,414 3 View

Why do I see variability in mRNA expression in qPCR of the same gene (ubiquitin)?

Hi everyone! I use HaCaT cells and i transfect them with siRNA. After 48 h I extract proteins with Laemmli and RNA with trizol. I proceed with western blot to check proteins levels (and they are...

26 January 2021 5,738 3 View

Which cell lines used as alternative to immortalized human keratinocyte cell lines (HaCaT) to asses antipsoriatic activity ?

suggest alternative cell lines for HaCaT cell lines for the assessment of antipsoriatic activity

31 December 2019 1,558 3 View

Where i can get HaCaT cells (as an immortalized human keratinocyte cell line) ?

for checking antipsoriatic activity

10 November 2019 745 1 View

Why my HaCaT cells die after thawed?

I tried to thaw my HaCaT cells several times but 99% of them died. They were frozen with 10% DMSO + 10% FBS + 80% DMEM. I also tried 10% DMSO + 90% FBS then they died less but still only 10-20%...

18 October 2019 1,762 2 View

Does transfection efficiency increase in the basal transformed HaCaT (Human Keratinocyte) cell line?

17 October 2019 2,484 1 View

How to resolve issue background fluorescence during Immunofluorescence (IF) studies of Hacat cells and Choroid Retinal Endothelial cells (RF/6A)?

Hello, I am studying cytokeratin 14 (K14) expression in Hacat human keratinocyte cell line and also von Willebrand factor (vWF) expression in choroid retinal endothelial cells (RF/6A). RF/6A cell...

09 October 2019 1,603 4 View

Does transfection efficiency increase in the basal transformed HaCaT (Human Keratinocyte) cell line?

09 October 2019 6,875 1 View

How can I hydrolyses my DNA extraction?

I'm trying to hydrolyses my DNA that I extracted from HaCat cells and analyse by HPLC. When I proceed the protocol with a control sample (Sigma Calf DNA) I can see the 4 bases in the UV spectrum,...

07 August 2019 5,231 3 View

Detecting large proteins through western-blot (ATM 350kDa)?

Hi everyone! I am trying to detect ATM expression in Hacat cells through western-blot. ATM is a large protein (350kDa) and I have tried running 60ug of lysate - 6% polyacrylamide gel for 3.5h...

05 June 2019 6,711 2 View

Can we use skin keratinocyte (HaCaT cell line) to assess cytotoxicity of extract using SRB method?

SRB assay has been used with cancer cell lines to assess anti-cancer activity of extract, but is it correct to apply this SRB assay on skin keratinocyte to assess cytotoxicity?

07 May 2019 665 1 View