i am looking for a protocol to isolate cytosol and membranes from cultured primary neurons
I need to do immunohistochemistry in mouse brain, particularly in neurotensin-cre mouse line.
22 May 2024 3,565 7 View
Dear experts, I am looking for someone interested in determining and possibly describing the new species in the photo (probably a Hirsutiella sp., Arachnida, Trombidiformes, Trombiculidae). The...
27 March 2024 9,244 2 View
Research publication details and processes
20 March 2024 3,140 2 View
Dear all, I am trying to functionalize crosslinked PVA by esterification using Mukaiyama reagent (Please see scheme attached). I have tried Dimethylcarbonate as a solvent, but PVA did not swell...
23 January 2024 2,381 1 View
Dear colleagues, could someone tell me the cut-off points of the Buss and Perry Aggression Questionnaire to distinguish between healthy and pathological subjects?
07 January 2024 890 2 View
The research question can be related to the vc world and innovation or also related to the silicon valley and gcc region. do you have interesting ideas?:)
19 September 2023 2,984 0 View
Hello, I am planning an experiment to measure enzimatic activity, and i will take sample every 24hrs. The only problem is that substrate is non soluble... nevertheless, enzyme can still access to...
21 June 2023 9,481 4 View
We want to study immune responses in mice treated with a lipophilic compound that has to be dissolved in DMSO. Given the toxicity and potential effect on immune response due to administration of...
14 June 2023 4,862 2 View
From an inclusion point of view
10 June 2023 9,477 0 View
Dear all, I am approaching EBV culturing, both clinical isolates and laboratory strains. I found different options for laboratory strains on ATCC (e.g., Strain HR1 (clone of P3) or Strain B95-8)....
16 May 2023 7,327 0 View
Vertebrates and invertebrates (e.g., arthropods: insects, crustaceans, etc.) have two different neuro-muscular systems given their differential construction: the former have an endoskeleton and...
07 August 2024 648 0 View
I am staining some brain sections stored in cryoprotectant that express a Histone H2B- GFP fusion protein that were generated ~10 years ago. I know I need to enhance signal with an anti-GFP...
07 August 2024 5,338 2 View
Firing in GABAergic neurons has been characterized in several areas of the brain, but patterns are the same?
04 August 2024 4,090 0 View
Hi, I am isolating monocytes from the bone marrow using the Mouse Monocyte EasySep kit. I want to treat these cells and monitor expression of specific markers over the course of 10 days. I will...
04 August 2024 7,282 2 View
I am trying to coat my micelles and PLGA based nanoparticles with cell membrane using an Avanti mini extruder according to literature . I use 200nm membrane but my particles end up to be 400nm And...
30 July 2024 3,294 3 View
Hi, I need to prepare a standard curve of H2O2 to evaluate the primary products of oxidation. H2O2 I have is 35%. How do I prepare a stock of H2O2 10 microlitere. If s.o has experience, I would be...
26 July 2024 3,460 2 View
Does it exist some special technique for cutting ceramic membranes for Cross-sectional FESEM or SEM images?
25 July 2024 5,482 2 View
I am starting to work on this question, with particular reference to what we might be able to learn from countries outside the UK. Of course this topic is huge, and I am breaking sustainability...
25 July 2024 1,545 3 View
While living in Boston, which has a fine metro system, there were times that I would exit from underground at the wrong location, thereby expecting a particular visual scene only to be momentarily...
17 July 2024 3,237 10 View
Hello, I am currently working with Malaria Pf/Pan nitrocellulose membranes coated for malaria detection. After drying the coated membranes in an Incubator (overnight at 37°C), I've noticed that...
16 July 2024 7,937 1 View