How to increase the final DNA yield using the Qiagen PowerSoil DNA extraction kit?

Hi, I am having troubles trying to extract DNA using the Qiagen PowerSoil kit from cave sediment (mud/clay). I always get very low yield:

03 January 2021 5,049 1 View

Can all iron oxide nanoparticles be considered superparamagnetic?

Dear researchers, Can all iron oxide nanoparticles be considered superparamagnetic? Or the "superparamagnetic" definition can be assigned only to certain types of iron oxide nanoparticles? If so,...

26 October 2020 546 5 View

Do you know how long it takes for a polyprepylene surgical mask to degrade?

I'm a master student in the university of Geneva. I have to find a scientific paper of the degradation of the surgical mask to add it in my master's thesis. I only found non-scientific journals...

03 September 2020 2,940 3 View

Piecewise multiplicative scatter correction in R?

Anyone is aware of an R package available online to perform a Piecewise Multiplicative Scatter Correction (PMSC)? This technique is an advance of the Multiplicative Scatter Correction (MSC)...

23 August 2020 2,893 2 View

What is the best chromatography (type, resin..) to separate blood lipoproteins among each other?

Dear all, I am trying to understand which is the "golden standard" method to separate lipoproteins from the blood (LDL, HDL, IDL, chylomicrons...). I found some methods online, and apparently...

10 August 2020 4,738 5 View

Latent Class/Profile Analysis AND prediction of binary outcome variable?

Hey everyone, I am trying to conduct a mixture of a latent class/ profile analysis because I have categorical and continuous outcome variables. (1) how can I combine these two? I would also...

26 March 2020 354 2 View

DdRAD final library shows low concentration at size selected peak. Anyone have any idea what could have gone wrong?

I sent off my first ddRAD library for sequencing and as QC it was first run through the bioanalyzer. I size selected with a pippin prep between 375-475 bp and the results of the bionalazyer show a...

12 January 2020 9,403 1 View

Special issue on social farming. Would do you like to contribute?

Dear all, I am Valentina Cattivelli and I am a guest editor at Sustainability (IF 2.5). With this, I would like to invite you to submit a paper within a special issue "Social farming for Social...

10 November 2019 5,392 13 View

Where to find good description of a cognitive and language development test standardization?

Hello everyone, I am new in the area of Developmental Psychology and I am looking for a good description of the standardization process of a test for cognitive and/or language development. For...

28 October 2019 2,346 4 View

How to separate human from bacterial RNA?

I extracted RNA from human saliva samples but the amount of bacterial (basically not human) RNA is very high: despite the high RNA concentration (Nanodrop quantification), after real-time PCR...

22 July 2018 4,414 3 View

Can we classify HV and LV lines based on line length?

I have dataset which shows the length of power lines. I need to classify the lines based on the line length. Is there a rule to classify the High voltage (HV) and low voltage (LV) lines based on...

03 March 2021 4,116 4 View

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02 March 2021 3,060 3 View

Should we change media after1st 6hrs after plating HUVEC bought from INVITROGEN?it's not recomended by invitrogen.Will DMSO present in stock matter?

We have HUVEC bought from Invitrogen. Cat no -C-003-5c. while first plating the cells we have bought the protocol suggests not to centrifuge the cell with media to remove the DMSO. Should we...

02 March 2021 3,713 2 View

Is There Any Feasible Method To Test Fluorescent Compounds Other Than Fluorescence Spectrometers ??

Is There Any Feasible Method To Test The Efficiency Of Fluorescent Compounds Other Than UV Spectrometers ? Suggestions Would Be Appreciated !

02 March 2021 5,785 3 View

New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before?

Question to you and THEM, the New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before? There appears to be a core problem for...

02 March 2021 3,024 2 View

How does one access the Cranet survey data on HRM practices?

Hello, The Cranet website does not provide any lead to the data obtained from their multinational survey on HRM practices. Any help on accessing the data would be most appreciated. Thank...

01 March 2021 9,536 3 View

Is there any research regarding how training and development is going to change in the era of Fourth Industrial Revolution?

As the organizations getting prepared for the fourth industrial revolution, to enhance the skills of their workforce what type of changes are necessary and going to occur in the training and...

01 March 2021 3,199 3 View

Does anyone know HRM channel in a 2 plex QIAGEN Rotor- Gene Q qPCR can be used as a separate channel?

I am going to have 3 different probes in my qPCR work that I am going to do. But I realized that the machine we have in the lab is a Rotor-Gene Q 2plex HRM Platform, saying it has green, yellow,...

01 March 2021 8,544 1 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Samples to study circular dichroism (chirality)?

I want to build a low-cost polarimeter to study circular dichroism (chirality). What type of samples can I use? I am looking for samples that can be found outside the laboratory.

01 March 2021 9,101 3 View