We want to study immune responses in mice treated with a lipophilic compound that has to be dissolved in DMSO.
Given the toxicity and potential effect on immune response due to administration of high doses of DMSO, we would like to reduce DMSO administration to the minimum.
To do so we would dissolve our lipophilic compound in a 10X less volume of DMSO than the one recommended by SDS. The preparation apparently look soluble but when further diluting with Physiologic solution it becomes a bit 'sandy', thus suggesting the compound is not fully dissolved..
Would the administration of such preparation (likely non solubilised) still be absorbed when injected peritoneally in mouse?
Being a lipophilic compound is it necessary to have it dissolved in solution in order to be absorbed into the circulation?
Thanks!
Dear Valentina,
Have you considered using a medium other than DMSO? I have seen studies using extra virgin olive oil as the vehicle for i.p injections of lipophilic compounds. It is far less toxic and much more can be safely administered. However it's important to note that there may be some immune effects from olive oil injections (especially since you are studying immune responses).
I hope this helps a little
Sarivin Vanan Thanks that's a good point.
I have to look better into this, but could be worth trying!!