What recipe would you recommend for the culturing medium of primary murine hepatocytes?

Hi, I want to test the growth factors on primary murine hepatocytes. Which kind of recipe I should use to avoid the endogenous growth factors existing in the medium/FBS/Supplements? e.g. This is...

09 October 2014 3,003 3 View

ChIP-qPCR?

Recently I am doing CHIP-qPCR to test one TF binding. I use 10% of sample as Input and the rest 90% to do IP. I have the TF-positive cell line A and TF-depleted cell line B. Primer is flanking...

05 June 2014 801 3 View

p300 & H3k4m1 antibody recommendation

I am doing ChIP-seq on mouse liver tissue with p300 & H3k4m1 antibodies. However, the antibodies from SC and Abcam do not work very well [these antibodies are used by other group]. I am wondering...

31 December 2012 4,813 0 View

How to get rid of free/unbound nuclear TF when immunoprecipitating a TF?

When we IP a transcription factor in the nuclear extract, we IP the DNA-associated TF and free TF. The DNA-bound TF is supposed to be more meaningful than free TF in the nucleus and cytoplasm. (Is...

07 August 2012 3,966 5 View

ChIP-seq library preparation

In regular ChIP-seq library preparation, my lab use 10ng as starting materials. Now I am studying a novel TF which just binds to several promters. This TF only transactivates 3-5 genes based on...

07 August 2012 8,806 3 View

Which kind cell lines are best cultured in suspension, especially hepatocytes?

I need a lot of cells and I am hesitating in choosing which kind of cell line I should use. It is better if it can be cultured in suspension. At the same time, it is biological interesting. I know...

06 July 2012 5,158 3 View

No title

02 March 2021 3,060 3 View

New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before?

Question to you and THEM, the New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before? There appears to be a core problem for...

02 March 2021 3,024 2 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Could you please recommend some good journal that accept very long revie papers (approximately 6000+ words) in neurosurgery, cancer biology ?

Could you please recommend some good journal that accept very long revie papers (approximately 6000+ words) in neurosurgery, cancer biology ?

01 March 2021 8,087 3 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

Do you need the CMV enhancer before the CMV promotor for a proper function of CMV on the gene that follows?

I'm a student and I have to produce a cell line with a knockin for the NRF2 gene with GFP. I have to put a promotor in front of the GFP because the gene will be too far away from the promotor of...

28 February 2021 7,127 2 View

Where to find data on trees in tropical agroforestry?

Hi, I'm looking for data (mainly related to management: growth rate, canopy size, soil and climate preferences, etc.) about tropical trees used in tropical agroforestry. Have you ever heard about...

28 February 2021 7,356 8 View

What could be some reasons why unstimulated monocytes are getting activated?

Im doing PBMC isolation -> CD14+ enrichment using magnetic beads -> stimulation setup. My negative control is just cells in cRPMI but they seem to get activated over and over again.

28 February 2021 7,883 3 View