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Questions related from Kezhuo Zhang
Hi, I want to test the growth factors on primary murine hepatocytes. Which kind of recipe I should use to avoid the endogenous growth factors existing in the medium/FBS/Supplements? e.g. This is...
10 October 2014 3,062 3 View
Recently I am doing CHIP-qPCR to test one TF binding. I use 10% of sample as Input and the rest 90% to do IP. I have the TF-positive cell line A and TF-depleted cell line B. Primer is flanking...
06 June 2014 915 3 View
I am wondering if there is any way to grow Flp-In Trex 293 cells (from Invitrogen) in suspension? Flp-In Trex 293 is adapted from HEK293 cell.
02 February 2014 6,611 4 View
I am doing ChIP-seq on mouse liver tissue with p300 & H3k4m1 antibodies. However, the antibodies from SC and Abcam do not work very well [these antibodies are used by other group]. I am wondering...
01 January 2013 4,847 0 View
When we IP a transcription factor in the nuclear extract, we IP the DNA-associated TF and free TF. The DNA-bound TF is supposed to be more meaningful than free TF in the nucleus and cytoplasm. (Is...
08 August 2012 4,021 5 View
In regular ChIP-seq library preparation, my lab use 10ng as starting materials. Now I am studying a novel TF which just binds to several promters. This TF only transactivates 3-5 genes based on...
08 August 2012 8,857 3 View
I need a lot of cells and I am hesitating in choosing which kind of cell line I should use. It is better if it can be cultured in suspension. At the same time, it is biological interesting. I know...
07 July 2012 5,203 3 View
