Dear all,
I am looking for some help regarding DNA contamination.
Before inject my protein sample and I normally treat them with Benzonase, but these days the protein recovery from gel gelfiltration got really reduced and I assume there should be short pieces of DNA should be inside them column thats why I can't recover my protein completely.
I tried 1.2 M Nacl wash, 0.5M NaOH wash and also tried pepsin digestion to recover my column
The pressure is normal but still I can't recover my protein completely, There is no buffer exchange during the purification step so my protein should not precipitate inside the column.
Could you suggest to get rid of short pieces of DNA in the gel filtration column?
Sonicate the sample before the gel filtration to shear the DNA.
Yes I will include sanitation step in my future experiments before injecting the sample. Currently my concern is How can I get rid of the short DNA contamination in the column? where my recovery is getting low
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